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BIN2A-c011
 
Resource Report
Resource Website 		RRID:Addgene_53657 BIN2A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AVM/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments. K241R 2026-08-15 01:16:34 0
CDC42BPAA-c004
 
Resource Report
Resource Website 		RRID:Addgene_53658 CDC42BPAA Other Kanamycin C-terminal His, TEV-cleavable, for LIC cloning. EXP-10-AE0544 http://www.thesgc.org/structures/4AW2/ Backbone Marker:SGC; Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
ETV1A-c014
 
Resource Report
Resource Website 		RRID:Addgene_53655 ETV1A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AVP/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
SNX3A-c018
 
Resource Report
Resource Website 		RRID:Addgene_53665 SNX3A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2YPS/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
SNX27A-c006
 
Resource Report
Resource Website 		RRID:Addgene_53662 SNX27A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4HAS/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
TSTA3A-c013
 
Resource Report
Resource Website 		RRID:Addgene_53663 TSTA3A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4B8Z/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
NEK1A-c011
 
Resource Report
Resource Website 		RRID:Addgene_53661 NEK1A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4APC/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
SNX14B-c101
 
Resource Report
Resource Website 		RRID:Addgene_53668 SNX14B Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BGJ/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
SOCS4B-c002
 
Resource Report
Resource Website 		RRID:Addgene_53669 SOCS4B Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2IZV/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
FEVA-c012
 
Resource Report
Resource Website 		RRID:Addgene_53666 FEVA Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/2YPR/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
MYO5BA-c002
 
Resource Report
Resource Website 		RRID:Addgene_53667 MYO5BA Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4J5M/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
CDKL5A-c014
 
Resource Report
Resource Website 		RRID:Addgene_53686 CDKL5A Other Ampicillin Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/4BGQ/ Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin Activating mutations T169D / Y171E 2026-08-15 01:16:33 0
SOCS2A-c016
 
Resource Report
Resource Website 		RRID:Addgene_53689 SOCS2A Other Ampicillin T7-lacl regulated, N-terminal his tag, TEV cleavage, LIC cloning using BseRI/ T4 pol, pET backbone. http://www.thesgc.org/structures/2C9W/ (complex) Backbone Marker:SGC; Vector Backbone:pLIC-SGC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:33 0
pACλCI-β-flap (831-1057)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_53733 λCI-β-flap fusion Other Chloramphenicol PMID:9121589 Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:34 1
pCS-membrane-ceruleanFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_53749 2x membrane linker Other Ampicillin PMID:19378112 Use NotI/SP6 for RNA to inject. Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean. This puts membrane-cerulean between XbaI and SnaBI of pCS. Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:33 2
mEmerald-Rab5a-7
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_54243 Rab5a Other Kanamycin . Excitation = 487; Emission = 509. Localization data: Ras GTPase Backbone Size:4750; Vector Backbone:mEmerald; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:38 4
mEmerald-Peroxisomes-2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_54228 Peroximal Targeting Signal 1 (PTS1) Other Kanamycin Peroximal Targeting Signal 1 (PTS1). Excitation = 487; Emission = 509. Localization data: Peroxisomes Backbone Size:4800; Vector Backbone:mEmerald-Peroxisomes; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:38 1
pBS SK mCherryROSAegfp
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_54322 mCherry and EGFP Other Ampicillin PMID:28421278 Backbone Size:2961; Vector Backbone:pBS SK(+); Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin 2026-08-15 01:16:39 1
LI A-C 35S c2
 
Resource Report
Resource Website 		RRID:Addgene_54409 cauliflower mosaic virus 35S promoter Other Gentamicin PMID:24551083 Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin 2026-08-15 01:16:40 0
LI A-B Ubi (+ intron) c4
 
Resource Report
Resource Website 		RRID:Addgene_54408 L. japonicus polyubiquitin promoter Other Gentamicin PMID:24551083 Backbone Marker:Parniske lab; Vector Backbone:pUC57(Gent); Vector Types:cloning vector; Bacterial Resistance:Gentamicin 2026-08-15 01:16:40 0

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