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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Sc RFC[2 + 3(GAT/SAC) + 4]-pET Resource Report Resource Website |
RRID:Addgene_258197 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | ||
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Sc RFC[2(SAC) + 3 + 4]-pET Resource Report Resource Website |
RRID:Addgene_258199 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | RFC2 (R183A) (SAC) | Addgene | 2026-09-26 02:55:07 | 0 | |
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Sc RFC[2 + 3 + 4(GAT)]-pET Resource Report Resource Website |
RRID:Addgene_258190 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | ||
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Sc RFC[2 + 3(SAC) + 4(SAC)]-pET Resource Report Resource Website |
RRID:Addgene_258193 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | ||
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Sc RFC[2 + 3 + 4(GAT/SAC)]-pET Resource Report Resource Website |
RRID:Addgene_258191 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | ||
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Sc RFC[2 + 3(SAC) + 4]-pET Resource Report Resource Website |
RRID:Addgene_258192 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | ||
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Sc Top2-FLAG-pRS402/GAL-L Resource Report Resource Website |
RRID:Addgene_257905 | Sc Top2 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:pRS402/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | |||
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Sc FLAG-Top1-pRS404/GAL-L Resource Report Resource Website |
RRID:Addgene_257904 | Sc Top1 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:pRS404/GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | |||
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Sc Pol31-pET(16b) (no tag) Resource Report Resource Website |
RRID:Addgene_257902 | Sc Pol31 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:07 | 0 | |||
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Sc RFC[2(SAC) + 3(SAC) + 4]-pET Resource Report Resource Website |
RRID:Addgene_258201 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | RFC2 (R183A) (SAC) | Addgene | 2026-09-26 02:55:07 | 0 | |
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Sc RFC[2(SAC) + 3(SAC) + 4(SAC)]-pET Resource Report Resource Website |
RRID:Addgene_258202 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | RFC2 (R183A) (SAC) | Addgene | 2026-09-26 02:55:07 | 0 | |
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Sc RFC5(SAC)-pCDFDuet Resource Report Resource Website |
RRID:Addgene_258210 | Sc RFC5 (mutant) | Saccharomyces cerevisiae | Streptomycin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin | RFC5 (K184A) (SAC) | Addgene | 2026-09-26 02:55:11 | 0 | |
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Sc RFC[2(GAT) + 3(SAC) + 4]-pET Resource Report Resource Website |
RRID:Addgene_258206 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | RFC2 (K71A) (GAT) | Addgene | 2026-09-26 02:55:11 | 0 | |
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Sc RFC[2(GAT) + 3 + 4(SAC)]-pET Resource Report Resource Website |
RRID:Addgene_258205 | RFC2 | Saccharomyces cerevisiae | Ampicillin | Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended. | Vector Backbone:pET(11a) / pET(16b); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | RFC2 (K71A) (GAT) | Addgene | 2026-09-26 02:55:14 | 0 | |
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Sc RFC[hk-1s + 5(SAC)]-pLANT/RIL Resource Report Resource Website |
RRID:Addgene_258209 | RFC1 | Saccharomyces cerevisiae | Kanamycin | Mix & Match (co-transform) with different versions of Sc RFC[2+3+4], either wild-type or containing various mutations. | Vector Backbone:pLANT(3b)/RIL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | N-terminally truncated | Addgene | 2026-09-26 02:55:14 | 0 | |
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YIplac211-VPS10-FRBx2-HA-msGFPx3 Resource Report Resource Website |
RRID:Addgene_255062 | Vps10-FRBx2-HA-msGFPx3 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:25 | 0 | |||
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YIplac211-VPS52-FRBx2-HA Resource Report Resource Website |
RRID:Addgene_255065 | Vps52-FRBx2-HA | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:25 | 0 | |||
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YIplac211-VPS52-msGFP2x2 Resource Report Resource Website |
RRID:Addgene_255067 | Vps52-msGFP2x2 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:25 | 0 | |||
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pML104-KanMX-sgRNAv2 Resource Report Resource Website |
RRID:Addgene_254712 | YKU70 sgRNA | Saccharomyces cerevisiae | Ampicillin and Kanamycin | PMID:42519825 | This plasmid contains an example guide sequence targeting **YKU70**. As described in our protocol paper, the guide sequence can be easily changed by designing forward and reverse primers for a new target and then using PCR and seamless plasmid recircularization. | Backbone Marker:John Wyrick lab; Backbone Size:11240; Vector Backbone:pML104; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | Addgene | 2026-09-26 02:55:32 | 0 | |
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pART7::COX:PtGFP Resource Report Resource Website |
RRID:Addgene_258581 | PtGFP | Saccharomyces cerevisiae | Ampicillin | PMID:16600023 | pART7::COX:PtGFP is for targeting PtGFP to the plant mitochondria matrix. The PtGFP gene is concatenated downstream of a sequence coding for the signal sequence (first 29 aminoacids) of the cytochrome oxidase subunit IV from yeast (COX-IV; Gene Bank X01418; Maarse et al. 1984, doi.org/10.1002/j.1460-2075.1984.tb02216.x; Akashi et al. 1998, doi.org/10.1016/S0014-5793(98)00717-0; Filipin et al. 2005, doi.org/10.1016/j.ceca.2004.08.002; Llopis et al. 1998, doi.org/10.1073/pnas.95.12.6803 ). A 5'UTR 144 nucleotide translation leader sequence from the tobacco etch virus (TEV; Gene Bank GU734657) was prepended for enhancement of expression (Carrington and Freed 1990 doi.org/10.1128/jvi.64.4.1590-1597.1990; Zeenko and Gallie 2005, DOI: 10.1074/jbc.M503576200; Peeters et al. 2000, doi.org/10.1007/s002390010044; Harper et al. 1998, doi.org/10.1074/jbc.273.2.1099). PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695). | Backbone Marker:Andrew Gleave (Addgene #247985); Backbone Size:5042; Vector Backbone:pART7; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:55:33 | 0 |
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