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Species: Saccharomyces cerevisiae
Genetic Insert: AVT1
Vector Backbone Description: Backbone Marker:Dan Gottschling (Addgene plasmid# 41894); Backbone Size:7882; Vector Backbone:pAG306-GPD-ccdB chr I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172144
Proper citation: RRID:Addgene_41898 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mediator of the Replication Checkpoint
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6021; Vector Backbone:pRS415; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12865299
Comments: There is a D304N mutation in LEU, relative to aa1 in LEU, it is not thought to affect function.
Proper citation: RRID:Addgene_41924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG18
Vector Backbone Description: Backbone Size:6112; Vector Backbone:Ycplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Comments: ATG18 was cloned with 300bp upstream and downstream of the gene. The myc tag was added later by mutagenesis.
Proper citation: RRID:Addgene_42530 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsv2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHIS2 parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704557
Proper citation: RRID:Addgene_42524 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kex2 protease
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5140; Vector Backbone:pcDNA6/V5-His Version C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23205847
Proper citation: RRID:Addgene_42504 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: N-myristoyl tranferase
Vector Backbone Description: Backbone Marker:Novagen/EMD Millipore; Backbone Size:3600; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23027949
Comments: Used for production of myristylated HIV-1 Gag in E. coli.
Proper citation: RRID:Addgene_42578 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Comments: The following mutations were found during the Addgene QC sequencing: Q31 deletion, S60G and Q68H.
Proper citation: RRID:Addgene_43909 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4/DBD (1-147)
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44014 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Proper citation: RRID:Addgene_43910 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Proper citation: RRID:Addgene_43911 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DIF1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18851834
Proper citation: RRID:Addgene_43912 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4/DBD (1-147)
Vector Backbone Description: Backbone Size:8600; Vector Backbone:N103; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22704655
Proper citation: RRID:Addgene_44176 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg17
Vector Backbone Description: Backbone Size:5756; Vector Backbone:pJK59; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23219485
Comments: Constructed by amplifying Atg17 with 300bp 5' of the start site.
Proper citation: RRID:Addgene_44175 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Backbone Marker:ATCC, Number: 77142; Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23222782
Proper citation: RRID:Addgene_40276 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6512; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_40610 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Miller et al (1998) Nucleic Acids Res 26(15):3577-3583 ; Backbone Size:6518; Vector Backbone:pBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_40600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Met3p-mCherry-Tub1
Vector Backbone Description: Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21294277
Proper citation: RRID:Addgene_37554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ras2 C-term (last 9 aa)
Vector Backbone Description: Backbone Size:4749; Vector Backbone:pKT0128; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755860
Proper citation: RRID:Addgene_37557 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tandem PA-GFP
Vector Backbone Description: Backbone Size:4882; Vector Backbone:pDH3; Vector Types:Yeast tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18727145
Proper citation: RRID:Addgene_37548 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38228 Copy
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