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Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-PGK1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), PGK1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1370 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA4-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: SSA4 promoter and SSA4 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1371 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5312; Vector Backbone:pET28a derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Comments: Construct is expressed with a precission protease cleavable 6xHis tag
Proper citation: RRID:Addgene_13727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeTadA1.0-T7 RNAP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:ColE1/AMP; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707425
Comments: Depositor confirms E366G does not affect plasmid function.
Proper citation: RRID:Addgene_137735 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25-U1A-PGK1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: RPL25 promoter and RPL25 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), PGK1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1375 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NPL3
Vector Backbone Description: Backbone Size:5181; Vector Backbone:YEp352; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8675010
Comments: ScaI fragment of NPL3 cloned into YEp352 SmaI site.
Proper citation: RRID:Addgene_1378 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal-Clb2-GFP
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS1304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171327
Comments: pPS1304 has the GAL1 promoter (EcoRI/XbaI fragment) and GFP (HindIII fragment) in YEp352 (2 micron ori).
Proper citation: RRID:Addgene_1381 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4-NLS
Vector Backbone Description: Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22366721
Proper citation: RRID:Addgene_138217 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Booster-PKA
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
Proper citation: RRID:Addgene_138373 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Booster-ERK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
Proper citation: RRID:Addgene_138375 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Booster-ROCK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
Proper citation: RRID:Addgene_138377 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GCN4
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138620 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TY5
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CBF1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TYE7
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138617 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GCR2
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138619 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 S389,398 A
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129485 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 S389,398 D
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129486 Copy
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