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Species: T. brucei
Genetic Insert: ADAT3 & ADAT2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17483465
Proper citation: RRID:Addgene_15917 Copy
Species: Nicotiana benthamiana
Genetic Insert: NbPT5b Promoter
Vector Backbone Description: Backbone Marker:47997 (Sylvestre Marillonnet); Backbone Size:2251; Vector Backbone:pICH41295; Vector Types:Bacterial Expression, pUC19-derived; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34260583
Proper citation: RRID:Addgene_159416 Copy
Species: Medicago truncatula
Genetic Insert: MtPT4 Promoter
Vector Backbone Description: Backbone Marker:47997 (Sylvestre Marillonnet); Backbone Size:2251; Vector Backbone:pICH41295; Vector Types:Bacterial Expression, pUC19-derived; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34260583
Proper citation: RRID:Addgene_159414 Copy
Species: Taxus canadensis
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:6200; Vector Backbone:psc101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30584096
Comments: Based on pAC-LYCipi (Addgene plasmid # 53279 ; http://n2t.net/addgene:53279 ; RRID:Addgene_53279), with backbone changed to pSC101, and crtE swapped for ggpps from Taxus canadensis (truncated & codon optimized).
Please note: The plasmid contains a 387 nucleotide deletion from Trp249 of Eh-idi to His30 of IDI-2 (when compared to the depositor's sequence) combining the two ORFs into a single ORF. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_122017 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Backbone Size:6049; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31548010
Proper citation: RRID:Addgene_122000 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122135 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122138 Copy
Genetic Insert: FBFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30633862
Proper citation: RRID:Addgene_122137 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30525474
Proper citation: RRID:Addgene_122186 Copy
Species: Homo sapiens
Genetic Insert: SCAF4
Vector Backbone Description: Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31104839
Proper citation: RRID:Addgene_122464 Copy
Species: Homo sapiens
Genetic Insert: SCAF4 CRISPR resistant
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31104839
Proper citation: RRID:Addgene_122465 Copy
Species: Homo sapiens
Genetic Insert: SCAF8 isoform D
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31104839
Proper citation: RRID:Addgene_122467 Copy
Species: Homo sapiens
Genetic Insert: SCAF8 isoform D CRISPR resistant
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31104839
Proper citation: RRID:Addgene_122468 Copy
Vector Backbone Description: Backbone Marker:Jan-Willem Veening; Backbone Size:3245; Vector Backbone:pPEPX; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31121970
Proper citation: RRID:Addgene_122631 Copy
Vector Backbone Description: Backbone Marker:Jan-Willem Veening; Backbone Size:3245; Vector Backbone:pPEPX; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31121970
Proper citation: RRID:Addgene_122632 Copy
Species: Synthetic
Genetic Insert: 35S:MS2-VP64:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-VP64 coding sequence = 1542-2195bp
RBCS terminator = 2203-2848bp
BsaI site #1 = 2883-2888bp
BsaI site #2 = 2897-2902bp
Proper citation: RRID:Addgene_122860 Copy
Species: Synthetic
Genetic Insert: 35S:MS2-SRDX:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-SRDX coding sequence = 1542-2069bp
RBCS terminator = 2077-2722bp
BsaI site #1 = 2757-2762bp
BsaI site #2 = 2771-2776bp
Proper citation: RRID:Addgene_122862 Copy
Species: Synthetic
Genetic Insert: pSUC2:MS2-VP64:tRBCS
Vector Backbone Description: Backbone Size:2728; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
SUC2 promoter = 644-2773bp
MS2-VP64 coding sequence = 2808-3461bp
RBCS terminator = 3469-4114bp
BsaI site #1 = 4149-4154bp
BsaI site #2 = 4163-4168bp
Proper citation: RRID:Addgene_122861 Copy
Species: Synthetic
Genetic Insert: 35S:MS2-G9a:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-G9a coding sequence = 1542-2885bp
RBCS terminator = 2893-3538bp
BsaI site #1 = 3573-3578bp
BsaI site #2 = 3587-3592bp
Proper citation: RRID:Addgene_122864 Copy
Species: Synthetic
Genetic Insert: 35S:MS2-p300:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-p300 coding sequence = 1542-3881bp
RBCS terminator = 3889-4534bp
BsaI site #1 = 4569-4574bp
BsaI site #2 = 4583-4588bp
Proper citation: RRID:Addgene_122863 Copy
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