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  • Bacterial Resistance:spectinomycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCDFDuet-1::ADAT3-ADAT2
 
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1+ mentions
RRID:Addgene_15917 ADAT3 & ADAT2 T. brucei Spectinomycin PMID:17483465 Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:08:27 1
pL0-NbPT5bPro
 
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RRID:Addgene_159416 NbPT5b Promoter Nicotiana benthamiana Spectinomycin PMID:34260583 Backbone Marker:47997 (Sylvestre Marillonnet); Backbone Size:2251; Vector Backbone:pICH41295; Vector Types:Bacterial Expression, pUC19-derived; Bacterial Resistance:Spectinomycin 2026-08-15 01:08:27 0
pL0-MtPT4Pro
 
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RRID:Addgene_159414 MtPT4 Promoter Medicago truncatula Spectinomycin PMID:34260583 Backbone Marker:47997 (Sylvestre Marillonnet); Backbone Size:2251; Vector Backbone:pICH41295; Vector Types:Bacterial Expression, pUC19-derived; Bacterial Resistance:Spectinomycin 2026-08-15 01:08:27 0
p5T7-LYCipi-ggpps
 
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RRID:Addgene_122017 GGPP synthase Taxus canadensis Spectinomycin PMID:30584096 Based on pAC-LYCipi (Addgene plasmid # 53279 ; http://n2t.net/addgene:53279 ; RRID:Addgene_53279), with backbone changed to pSC101, and crtE swapped for ggpps from Taxus canadensis (truncated & codon optimized). Please note: The plasmid contains a 387 nucleotide deletion from Trp249 of Eh-idi to His30 of IDI-2 (when compared to the depositor's sequence) combining the two ORFs into a single ORF. This mutation is not known to affect plasmid function. Backbone Size:6200; Vector Backbone:psc101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin codon optimized, truncated first 98 amino acids, methionine added. Please see Depositor Comments 2026-08-15 01:03:40 0
pSGAb-spe
 
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1+ mentions
RRID:Addgene_122000 none Other Spectinomycin PMID:31548010 Backbone Size:6049; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:40 1
pROP-PP-GFP
 
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1+ mentions
RRID:Addgene_122135 GFP Spectinomycin PMID:30633862 Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:42 1
pROP-IP-FBFP
 
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1+ mentions
RRID:Addgene_122138 FBFP Spectinomycin PMID:30633862 Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:42 1
pROP-PP-FBFP
 
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RRID:Addgene_122137 FBFP Spectinomycin PMID:30633862 Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:42 0
pCpf1-sp
 
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1+ mentions
RRID:Addgene_122186 Spectinomycin PMID:30525474 Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:42 1
pDONR223 SCAF4
 
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RRID:Addgene_122464 SCAF4 Homo sapiens Spectinomycin PMID:31104839 Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:44 0
pDONR223 SCAF4_CRISPR resistant
 
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RRID:Addgene_122465 SCAF4 CRISPR resistant Homo sapiens Spectinomycin PMID:31104839 Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Synonymous mutations conferring resistance to gRNAs described in Gregersen et al. 2026-08-15 01:03:44 0
pDONR223 SCAF8_isoD
 
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RRID:Addgene_122467 SCAF8 isoform D Homo sapiens Spectinomycin PMID:31104839 Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:44 0
pDONR223 SCAF8_isoD_CRISPR_resistant
 
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RRID:Addgene_122468 SCAF8 isoform D CRISPR resistant Homo sapiens Spectinomycin PMID:31104839 Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin Synonymous mutations conferring resistance to gRNAs described in Gregersen et al. 2026-08-15 01:03:44 0
pPEPX
 
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RRID:Addgene_122631 Spectinomycin PMID:31121970 Backbone Marker:Jan-Willem Veening; Backbone Size:3245; Vector Backbone:pPEPX; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:46 0
pPEPX-Plac
 
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1+ mentions
RRID:Addgene_122632 Spectinomycin PMID:31121970 Backbone Marker:Jan-Willem Veening; Backbone Size:3245; Vector Backbone:pPEPX; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:46 1
pMH204
 
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RRID:Addgene_122860 35S:MS2-VP64:tRBCS Synthetic Spectinomycin PMID:31557222 Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): 35S promoter = 656-1502bp MS2-VP64 coding sequence = 1542-2195bp RBCS terminator = 2203-2848bp BsaI site #1 = 2883-2888bp BsaI site #2 = 2897-2902bp Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:47 0
pJL009
 
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RRID:Addgene_122862 35S:MS2-SRDX:tRBCS Synthetic Spectinomycin PMID:31557222 Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): 35S promoter = 656-1502bp MS2-SRDX coding sequence = 1542-2069bp RBCS terminator = 2077-2722bp BsaI site #1 = 2757-2762bp BsaI site #2 = 2771-2776bp Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:48 0
pMH205
 
Resource Report
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RRID:Addgene_122861 pSUC2:MS2-VP64:tRBCS Synthetic Spectinomycin PMID:31557222 Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): SUC2 promoter = 644-2773bp MS2-VP64 coding sequence = 2808-3461bp RBCS terminator = 3469-4114bp BsaI site #1 = 4149-4154bp BsaI site #2 = 4163-4168bp Backbone Size:2728; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:48 0
pJL036
 
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RRID:Addgene_122864 35S:MS2-G9a:tRBCS Synthetic Spectinomycin PMID:31557222 Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): 35S promoter = 656-1502bp MS2-G9a coding sequence = 1542-2885bp RBCS terminator = 2893-3538bp BsaI site #1 = 3573-3578bp BsaI site #2 = 3587-3592bp Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:48 0
pJL024
 
Resource Report
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RRID:Addgene_122863 35S:MS2-p300:tRBCS Synthetic Spectinomycin PMID:31557222 Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): 35S promoter = 656-1502bp MS2-p300 coding sequence = 1542-3881bp RBCS terminator = 3889-4534bp BsaI site #1 = 4569-4574bp BsaI site #2 = 4583-4588bp Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:48 0

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