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Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi APOBEC1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69668 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi AID
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69667 Copy
Species: Other
Genetic Insert: dnaQ926 RBS+ dam seqA emrR ugi CDA1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69700 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA yffI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69673 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA rsmE
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69671 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdAB
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69678 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi CDA1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69677 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdAB(P334L)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69682 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA nrdA(A65V)B
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69680 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA emrR ugi CDA1 (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69689 Copy
Species: Other
Genetic Insert: dnaQ926 dam seqA ugi APOBEC (opt)
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:CloDF13; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26443021
Comments: The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
Proper citation: RRID:Addgene_69685 Copy
Species: Other
Genetic Insert: LdBPK_363130.1:MAC055-F03:C235029
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/5C4Q for more information
Proper citation: RRID:Addgene_69963 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Alexeyev Lab; Backbone Size:4064; Vector Backbone:pMA2008; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27136098
Proper citation: RRID:Addgene_70110 Copy
Species: Other
Genetic Insert: H1-Tet-sgrna cassette
Vector Backbone Description: Backbone Marker:David Baltimore; Backbone Size:9940; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732831
Proper citation: RRID:Addgene_70183 Copy
Species: Other
Genetic Insert: Photinus pyralis sulfotransferase 2
Vector Backbone Description: Backbone Marker:Produced in Jing-Ke Weng lab; Backbone Size:5350; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27227579
Comments: Expresses robustly in E.coli. Minimal activity for desulfonation of p-nitrophenolsulfate with PAP
Proper citation: RRID:Addgene_74122 Copy
Species: Other
Genetic Insert: Green Lantern Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4110; Vector Backbone:pSport I; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25448690
Proper citation: RRID:Addgene_74101 Copy
Species: Other
Genetic Insert: LacZ sgRNA
Vector Backbone Description: Backbone Marker:Moffat Lab; Vector Backbone:pLCKO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26627737
Proper citation: RRID:Addgene_74179 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74215 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_74214 Copy
Species: Other
Genetic Insert: Luciferase sgRNA
Vector Backbone Description: Backbone Marker:Moffat Lab; Vector Backbone:pLCKO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26627737
Proper citation: RRID:Addgene_74190 Copy
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