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Species: Drosophila melanogaster
Genetic Insert: miR-1003 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): miR-1003-psm (gagctcCTGTGAATATGTAAAAGAGTGAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17772 Copy
Species: Other
Genetic Insert: None
Vector Backbone Description: Vector Backbone:pUbC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177619 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 FSE
Vector Backbone Description: Vector Backbone:pUbC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34185680
Proper citation: RRID:Addgene_177618 Copy
Species: Mus musculus
Genetic Insert: anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2877233. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_177575 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV116; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177696 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (Mu variant)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34519517
Proper citation: RRID:Addgene_177578 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV116; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177699 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: SOUL
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32645306
Comments: SOUL is a combination of the stabilized step-function opsin (SSFO) mutations C128S and D156A with the T159C mutation in channelrhodopsin previously reported by Dr. Karl Deisseroth et. al.
Proper citation: RRID:Addgene_177577 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV066; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177698 Copy
Species: Mus musculus
Genetic Insert: Myc-T58A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7657; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28089889
Proper citation: RRID:Addgene_177648 Copy
Species: Patiria miniata
Genetic Insert: Dishevelled
Vector Backbone Description: Vector Backbone:pCS2+8; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739818
Proper citation: RRID:Addgene_177640 Copy
Species: Patiria miniata
Genetic Insert: Dishevelled
Vector Backbone Description: Vector Backbone:pCS2+8; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739818
Proper citation: RRID:Addgene_177642 Copy
Species: Patiria miniata
Genetic Insert: Rab7
Vector Backbone Description: Vector Backbone:pCS2+8; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739818
Proper citation: RRID:Addgene_177641 Copy
Species: Patiria miniata
Genetic Insert: Lamp1
Vector Backbone Description: Vector Backbone:pCS2+8; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739818
Proper citation: RRID:Addgene_177639 Copy
Species: Patiria miniata
Genetic Insert: Frizzled 1
Vector Backbone Description: Vector Backbone:pCS2+8; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34739818
Proper citation: RRID:Addgene_177638 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-miR-1006 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17760 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-miR-1003 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Minigenes containing mir-1003 and mir-1006 and flanking exons were PCR amplified from genomic DNA and cloned into pMT-puro.
Proper citation: RRID:Addgene_17761 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin and cloned into pMT-puro.
Proper citation: RRID:Addgene_17764 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-ps (gagctcACTATACAACCTACTACCTCAactagt) Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17766 Copy
Species: Drosophila melanogaster
Genetic Insert: dme-let-7 minigene
Vector Backbone Description: Backbone Marker:Carl Thummel Lab; Backbone Size:10200; Vector Backbone:pCaSpeR4-tubulin promoter; Vector Types:Drosophila P-element vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: let-7 was amplified from genomic DNA with primers 474 bp upstream and 310 bp downstream of the let-7 hairpin. pCaSpeR4 contains a ~2.4 kb tubulin promoter inserted between the EcoRI and KpnI sites.
Proper citation: RRID:Addgene_17769 Copy
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