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Species: Homo sapiens
Genetic Insert: adhesion regulating molecule 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16906146
Proper citation: RRID:Addgene_19417 Copy
Species: Homo sapiens
Genetic Insert: Heat Shock Factor 1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11486022
Comments: FLAG-tagged full length hHSF1 (aa1-529) was generated by subcloning the human HSF1-flag construct out of pCMV-HSF1-flag and into the pBABE/puro retroviral vector via the EcoRI site. (Kingston #1155)
Proper citation: RRID:Addgene_1948 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19637 Copy
Species: Mus musculus
Genetic Insert: MST1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12384512
Proper citation: RRID:Addgene_1965 Copy
Species: Caenorhabditis elegans
Genetic Insert: <*iA-1xNLS-GFP::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC292; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19646 Copy
Species: Homo sapiens
Genetic Insert: DNAJB6b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19528 Copy
Species: Homo sapiens
Genetic Insert: BAF170
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10078207
Comments: To make pFastBac1-BAF170, BAF170 sequences were transferred into pFastBac1 using NotI and HindIII. A C-terminal Flag epitope was added by PCR. The product of this PCR reaction was digested with SphI and HindIII, transferred into pFastBac1-BAF170. (kingston #1232)
Proper citation: RRID:Addgene_1955 Copy
Species: Homo sapiens
Genetic Insert: BRG1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11238380
Comments: Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 digested with SalI to liberate entire flag tagged BRG1 seq. (Kingston #1359)
Proper citation: RRID:Addgene_1959 Copy
Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19531 Copy
Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 9
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19532 Copy
Species: Homo sapiens
Genetic Insert: BAF155
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10078207
Comments: We constructed a composite full-length cDNA for hSWI3 (BAF155) using a 2.4 kb partial human cDNA that was isolated as described previously and a 1.8 kb RT-PCR-amplified fragment obtained from human liver poly(A) RNA. We consistently isolated the partial hSWI3 cDNA (2.4 kb) from several libraries. It appears that an adenine-rich stretch at the 3' end of these cDNAs hybridizes with the oligo(dt) primers used to create the first strand of the cDNA. An oligo that anneals in the sense direction to the partial cDNA, and an oligo that anneals to the 3' UTR of the highly related hSWI3 clone described previously, BAF155, were used to generate the missing coding sequences. The amplified fragment was inserted into the BamHI and SpeI sites of pBS-5'hSWI3, which contains the partial hSWI3 cDNA. The protein encoded by this complete ORF is highly related to BAF155, except for a few positions scattered throughout the protein. Most of these differences are substitutions of BAF155 sequences with the corresponding sequences of BAF170. To generate pFastBac1-BAF155, an XhoI
Proper citation: RRID:Addgene_1954 Copy
Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily A, member 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19545 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Marker:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Backbone Size:7519; Vector Backbone:MSCV P2Gm; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19751 Copy
Genetic Insert: oligo targeting Firefly luciferase
Vector Backbone Description: Backbone Marker:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Backbone Size:7518; Vector Backbone:MSCV P2Gm; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: MSCV-based entry vector for cloning and validating miR30-based RNAi constructs. The vector expresses puromycin-resistance and GFP, separated by the FMDV 2A peptide.
This is considered the "empty" vector and can be used for cloning. It contains a hairpin targeting Firefly luciferase.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19750 Copy
Species: Mus musculus
Genetic Insert: Nore1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081
Proper citation: RRID:Addgene_1975 Copy
Species: Homo sapiens
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:See author's map; Backbone Size:6000; Vector Backbone:MO91; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16921383
Comments: See Author's Map for empty backbone (MO91) information.
Proper citation: RRID:Addgene_19754 Copy
Species: Homo sapiens
Genetic Insert: MKK7B2Jnk1a1(APF)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12052897
Proper citation: RRID:Addgene_19730 Copy
Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16247032
Proper citation: RRID:Addgene_19699 Copy
Vector Backbone Description: Backbone Size:9205; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are minor discrepancies between Addgene's quality control sequence and the reference sequence provided by the depositing lab. These discrepancies should not affect plasmid function.
Proper citation: RRID:Addgene_19732 Copy
Species: Homo sapiens
Genetic Insert: Bax
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10228148
Proper citation: RRID:Addgene_19741 Copy
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