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Species: Synthetic
Genetic Insert: Coff/Fon-NpHR3.3-EYFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:AAV2-nEF-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
Proper citation: RRID:Addgene_137154 Copy
Species: Synthetic
Genetic Insert: Con/Fon-iC++-EYFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:AAV2-nEF-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
Proper citation: RRID:Addgene_137155 Copy
Species: Synthetic
Genetic Insert: Con/Fon-ChRmine-p2a-oScarlet
Vector Backbone Description: Backbone Size:4600; Vector Backbone:AAV2-nEF-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
Proper citation: RRID:Addgene_137159 Copy
Species: Synthetic
Genetic Insert: Coff/Fon-ChRmine-p2a-oScarlet
Vector Backbone Description: Backbone Size:4600; Vector Backbone:AAV2-nEF-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
Proper citation: RRID:Addgene_137160 Copy
Species: Synthetic
Genetic Insert: Con/Foff 2.0-ChR2-EYFP
Vector Backbone Description: Backbone Size:4600; Vector Backbone:AAV2-nEF-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
Proper citation: RRID:Addgene_137163 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:PUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31909199
Comments: The Addgene NGS result is not a full plasmid sequence and does not completely confirm the A/T rich Plasmodium cynomolgi strain M centromere sequence. A NotI restriction digest should be used to verify the plasmid, as an intact plasmid will yield 2 bands that add up to the full plasmid size of approximately 16 kb.
Proper citation: RRID:Addgene_137169 Copy
Species: Synthetic
Genetic Insert: Npu-ABEmax_N-terminal
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31937940
Proper citation: RRID:Addgene_137173 Copy
Species: Homo sapiens
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE-80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790548
Comments: Truncated form of SIRT1. Missing amino acids 6-83. Molecular Weight: 81681 Da.
Proper citation: RRID:Addgene_13735 Copy
Species: Synthetic
Genetic Insert: v5 AAV-CBE C-terminal; U6-protospacer
Vector Backbone Description: Vector Backbone:px601; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31937940
Comments: Clone in protospacer by cutting with BsmBI/Esp3I and ligating annealed oligos as described in methods. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.
Proper citation: RRID:Addgene_137176 Copy
Species: Synthetic
Genetic Insert: v5 AAV-CBE N-terminal; U6-protospacer
Vector Backbone Description: Vector Backbone:px601; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31937940
Comments: Clone in protospacer by cutting with BsmBI/Esp3I and ligating annealed oligos as described in methods. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.
Proper citation: RRID:Addgene_137175 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV bam neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16775354
Proper citation: RRID:Addgene_13733 Copy
Species: Synthetic
Genetic Insert: v5 AAV-CBE C-terminal; U6-protospacer
Vector Backbone Description: Vector Backbone:px601; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31937940
Comments: Clone in protospacer by cutting with BsmBI/Esp3I, gel-extracting product to remove RFP dropout cassette, and ligating annealed oligos as described in methods. Background colonies will be RFP+. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.
Proper citation: RRID:Addgene_137178 Copy
Species: Mus musculus
Genetic Insert: mouse Kif3A
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pGREENLANTERN-1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11102514
Proper citation: RRID:Addgene_13742 Copy
Species: Homo sapiens
Genetic Insert: SIRT7
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE-80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790548
Comments: Derived from pcDNA3.1 SIRT1–7 with a FLAG tag, obtained from E. Verdin (University of California, San Francisco). Molecular Weight: 44898 Da.
Proper citation: RRID:Addgene_13740 Copy
Species: Synthetic
Genetic Insert: CNC3 (CASANOVA-C3)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33330940
Comments: Please visit https://www.biorxiv.org/content/10.1101/858589v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137191 Copy
Species: Homo sapiens
Genetic Insert: EGFP-Rac1(wt)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11030651
Comments: Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Proper citation: RRID:Addgene_13719 Copy
Species: Homo sapiens
Genetic Insert: EGFP-Rac1(Q61L)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11030651
Comments: Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Proper citation: RRID:Addgene_13720 Copy
Species: Synthetic
Genetic Insert: Coff/Fon-sRGECO
Vector Backbone Description: Backbone Size:5300; Vector Backbone:AAV2-EF1a-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 1F GGGACGACATGACTTAACCAG; Intron 1R CCAGCCCTTCTCATGTTCAG
Proper citation: RRID:Addgene_137128 Copy
Species: Bacteriophage P1
Genetic Insert: Cre-GFP fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG.
GFP-tag was added at the C-terminus of Cre.
Cre-GFP fusion protein is localized in the cell nucleus.
Proper citation: RRID:Addgene_13776 Copy
Species: Bacteriophage P1
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG.
Myc-tag was added at the C-terminus of Cre.
Proper citation: RRID:Addgene_13775 Copy
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