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Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177772 Copy
Species: Homo sapiens
Genetic Insert: GRHL1
Vector Backbone Description: Vector Backbone:pcDNA3.1/Hygro(+) (Thermo Fisher Scientific, #V87020); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177773 Copy
Species: Homo sapiens
Genetic Insert: CopGFP inserted behind human synapsin I promoter
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34128504
Proper citation: RRID:Addgene_177810 Copy
Species: Arabidopsis thaliana
Genetic Insert: SNF1-related protein kinase 2.3
Vector Backbone Description: Backbone Marker:Life Sensors; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22160701
Proper citation: RRID:Addgene_177846 Copy
Species: Arabidopsis thaliana
Genetic Insert: SNF1-related protein kinase 2
Vector Backbone Description: Backbone Marker:Life Sensors; Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22160701
Proper citation: RRID:Addgene_177845 Copy
Species: Other
Genetic Insert: hFluc
Vector Backbone Description: Backbone Marker:Addgene #177714; Vector Backbone:phFluc-WT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34857875
Proper citation: RRID:Addgene_177727 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1-like 2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19898420
Proper citation: RRID:Addgene_177842 Copy
Species: Homo sapiens
Genetic Insert: Folate receptor alpha
Vector Backbone Description: Vector Backbone:pCDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23851396
Comments: IgK = Ig kappa leader, th = thrombin cleavage site, Fc = Igl Fc fragment
Proper citation: RRID:Addgene_177841 Copy
Vector Backbone Description: Backbone Size:10151; Vector Backbone:pDEST-DB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35137167
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177796 Copy
Vector Backbone Description: Backbone Size:10394; Vector Backbone:pDEST-AD-CYH2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35137167
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177795 Copy
Vector Backbone Description: Backbone Size:10164; Vector Backbone:pDEST-DB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35137167
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177798 Copy
Vector Backbone Description: Backbone Size:10388; Vector Backbone:pDEST-AD-CYH2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35137167
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453987v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_177797 Copy
Species: Synthetic
Genetic Insert: KRaION1
Vector Backbone Description: Backbone Size:4002; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35427452
Comments: Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
Proper citation: RRID:Addgene_177799 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177747 Copy
Species: Homo sapiens
Genetic Insert: BSD-based EXSISERS
Vector Backbone Description: Vector Backbone:pBlueScript-KS II (+); Vector Types:Mammalian Expression, CRISPR, Luciferase, Synthetic Biology, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34083785
Comments: HMGU has filed a patent (WO2020161236A1 “Detection of splice events of a gene of interest”) disclosing the capabilities of EXSISERS, and retains ownership rights to EXSISERS Material.
Proper citation: RRID:Addgene_177867 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7 seed mutant perfect site
Vector Backbone Description: Backbone Marker:David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Comments: Luciferase-reporter inserts were made by annealing oligonucleotides with their reverse complements, leaving overhangs for the indicated restriction sites (lower case): let-7-psm (gagctcACTATACAACCTACAAGCACAactagt). Annealed oligos were ligated into SacI/SpeI-cleaved pIS2. These plasmids were linearized with HindIII, polished with Klenow enzyme to create blunt ends, and digested with NotI to excise the Renilla luciferase gene with the modified UTR from the remainder of pIS2. The gel-purified Renilla gene fragment was then ligated into pMT-puro between EcoRV and NotI sites for copper-induced expression in S2 cells.
Proper citation: RRID:Addgene_17773 Copy
Species: Synthetic
Genetic Insert: pCMV(MinDis)-iCytSnFR_ER
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177742 Copy
Species: Homo sapiens
Genetic Insert: SMARCC1
Vector Backbone Description: Backbone Size:4775; Vector Backbone:pFAST BAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34857874
Proper citation: RRID:Addgene_177863 Copy
Species: Drosophila melanogaster
Genetic Insert: cg11094 utr fragment
Vector Backbone Description: Backbone Marker:pMT-puro from David Sabatini Lab; Backbone Size:0; Vector Backbone:pMT-puro with renilla luciferase; Vector Types:Insect Expression, Luciferase, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17589500
Proper citation: RRID:Addgene_17777 Copy
Species: Synthetic
Genetic Insert: iCytSnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34982029
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2021.10.04.463082v3.
Proper citation: RRID:Addgene_177738 Copy
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