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Species: T. brucei
Genetic Insert: Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
Vector Backbone Description: Backbone Marker:Cross Lab; Vector Backbone:pHD309; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23954366
Comments: For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html
There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab; most notably in the beta-tubulin ORF, HSV thymidine kinase and the T. brucei Aldolase 3'UTR. These mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_48359 Copy
Species: T. brucei
Genetic Insert: FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23954366
Comments: For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html
There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab. These mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_48367 Copy
Species: T. brucei
Genetic Insert: Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
Vector Backbone Description: Backbone Marker:Cross Lab; Vector Backbone:pHJ17; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23954366
Comments: For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html
There are several mismatches between Addgene's quality control sequence and the reference sequence from the depositing lab; most notably in the beta-tubulin ORF, HSV thymidine kinase and the T. brucei Aldolase 3'UTR. These mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_48361 Copy
Species: T. brucei
Genetic Insert: 3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Cre/Lox, Knockout in T. Brucei; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23954366
Comments: For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Proper citation: RRID:Addgene_48365 Copy
Species: Homo sapiens
Genetic Insert: hDDB1-BPB
Vector Backbone Description: Backbone Marker:pGex2T; Vector Backbone:pCool; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16413485
Comments: BL21 is recommended for protein overexpression.
Proper citation: RRID:Addgene_48639 Copy
Species: jellyfish
Genetic Insert: roGFP1
Vector Backbone Description: Backbone Size:3418; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23589496
Comments: This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene).
Proper citation: RRID:Addgene_48634 Copy
Species: jellyfish
Genetic Insert: roGFP1
Vector Backbone Description: Backbone Size:3418; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23589496
Comments: This plasmids needs to be amplified and maintained in M15 at all times as this strain has the pREP4 repressor vector that prevents undesired expression (it's important to maintain the plasmid when the protein is not expressed even if it's believed to be inert for the bacteria, to avoid selective pressure in favour of recombinant versions of the gene).
Proper citation: RRID:Addgene_48631 Copy
Species: Homo sapiens
Genetic Insert: DDB1
Vector Backbone Description: Backbone Marker:Pharmagen; Vector Backbone:pAcGHLT-B; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16413485
Proper citation: RRID:Addgene_48638 Copy
Vector Backbone Description: Backbone Size:5768; Vector Backbone:pCS2; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23667577
Proper citation: RRID:Addgene_48637 Copy
Vector Backbone Description: Backbone Size:5798; Vector Backbone:pCS2; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23667577
Proper citation: RRID:Addgene_48636 Copy
Species: Jellyfish
Genetic Insert: ERrefGFP1
Vector Backbone Description: Backbone Size:5393; Vector Backbone:pCDNA3 derivative; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23589496
Proper citation: RRID:Addgene_48635 Copy
Species: jellyfish
Genetic Insert: ERroGFP
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pcDNA3 derivative; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23589496
Proper citation: RRID:Addgene_48629 Copy
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23582324
Comments: Plasmid map is theoretical and may not represent actual sequence. The presence of important plasmid features have been verified by sequencing.
Proper citation: RRID:Addgene_48754 Copy
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23582324
Comments: Plasmid map is theoretical and may not represent actual sequence. The presence of important plasmid features have been verified by sequencing.
Proper citation: RRID:Addgene_48753 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:6600; Vector Backbone:Bluescript; Vector Types:Mammalian Expression, pPy; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23582324
Proper citation: RRID:Addgene_48759 Copy
Species: Mus musculus
Genetic Insert: Tfe3::ERT2
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23582324
Proper citation: RRID:Addgene_48756 Copy
Species: Mus musculus
Genetic Insert: Fbxl3
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6299; Vector Backbone:p3xFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17462724
Proper citation: RRID:Addgene_48751 Copy
Species: Mus musculus
Genetic Insert: truncated mPer2 promoter/enhancer region (-1128 to +1060)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15699353
Comments: Minor sequencing discrepancies may exist between the mPer2 promoter reference sequence and Addgene's sequencing results, but do not affect the function of the plasmid.
Proper citation: RRID:Addgene_48743 Copy
Species: Mus musculus
Genetic Insert: truncated mPer2 promoter/enhancer region (-1128 to +386)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15699353
Comments: Minor sequencing discrepancies may exist between the mPer2 promoter reference sequence and Addgene's sequencing results, but do not affect the function of the plasmid.
Proper citation: RRID:Addgene_48742 Copy
Species: Mus musculus
Genetic Insert: mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15699353
Comments: Minor sequencing discrepancies may exist between the mPer2 promoter reference sequence and Addgene's sequencing results, but do not affect the function of the plasmid.
Proper citation: RRID:Addgene_48748 Copy
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