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Species: Glycine max
Genetic Insert: soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5444; Vector Backbone:pET30Xa-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16157595
Comments: prepared by four sequential mutations of Cys to Ser, from pET30Xa-SBL1(160S,NoCys)
Proper citation: RRID:Addgene_66875 Copy
Genetic Insert: eGFP in a transposon
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3000; Vector Backbone:pBlunt-topo; Vector Types:source for a Tn7 transposon; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15317651
Proper citation: RRID:Addgene_66873 Copy
Genetic Insert: eYFP in a transposon
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3000; Vector Backbone:pBlunt-topo; Vector Types:source for a Tn7 transposon; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15317651
Proper citation: RRID:Addgene_66874 Copy
Species: Homo sapiens
Genetic Insert: Alpha-actinin 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23515331
Proper citation: RRID:Addgene_66935 Copy
Species: Xenopus laevis
Genetic Insert: His6-Thrombin-Xenopus laevis histones H2A
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22617796
Proper citation: RRID:Addgene_66890 Copy
Species: Discosoma
Genetic Insert: LOC8R-PSmOrange
Vector Backbone Description: Backbone Size:3965; Vector Backbone:pPSmOrange-N1; Vector Types:; Bacterial Resistance:Kanamycin
Comments: The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)
Proper citation: RRID:Addgene_66897 Copy
Species: Synthetic
Genetic Insert: LOC8R
Vector Backbone Description: Backbone Marker:GeneArt (LifeTechnologies); Backbone Size:2278; Vector Backbone:pMK-RQ (kanR); Vector Types:; Bacterial Resistance:Kanamycin
Comments: The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)
Insert into an N1 vector by subcloning. The number of Cox8 MTS repeats in LOC8R can be modified as follows:
4x Cox8: Insert at HindIII & AgeI sites. (subclone directly).
3x Cox8: Digest with BamHI and re-ligate. insert at HindIII & AgeI sites.
2x Cox8: Digest with XhoI and re-ligate. insert at HindIII & AgeI sites.
1x Cox8: Digest with HindIII and BamHI. Insert at HindIII & BamHI sites.
Proper citation: RRID:Addgene_66896 Copy
Species: Homo sapiens
Genetic Insert: Beta-2-adrenergic receptor-mCerulean
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3954; Vector Backbone:Derived from pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15297467
Comments: Reference for monomeric Cerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_66957 Copy
Species: Synthetic
Genetic Insert: ELP[V-30]
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5310; Vector Backbone:pET-24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26726995
Proper citation: RRID:Addgene_67014 Copy
Species: Mus musculus
Genetic Insert: Brsk1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4200; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19648910
Proper citation: RRID:Addgene_67155 Copy
Species: Mycobacterium smegmatis
Genetic Insert: fbiC gene from Mycobacterium smegmatis
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22175817
Comments: Gene is induced with IPTG. Following induction, Fo present in media.
Proper citation: RRID:Addgene_67283 Copy
Species: Homo sapiens
Genetic Insert: ARF1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67306 Copy
Species: Homo sapiens
Genetic Insert: ARF1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67307 Copy
Species: Homo sapiens
Genetic Insert: Beta-2-adrenergic receptor-mRFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3954; Vector Backbone:Derived from pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15297467
Proper citation: RRID:Addgene_67145 Copy
Species: Homo sapiens
Genetic Insert: ARL2
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67318 Copy
Species: Homo sapiens
Genetic Insert: ARL6
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67329 Copy
Species: Homo sapiens
Genetic Insert: ARL5B
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67326 Copy
Species: Homo sapiens
Genetic Insert: ARL4
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67322 Copy
Species: Homo sapiens
Genetic Insert: ARL4
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67323 Copy
Species: Homo sapiens
Genetic Insert: ARL3
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27057421
Proper citation: RRID:Addgene_67320 Copy
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