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Species: Discosoma sp
Genetic Insert: DsRed2
Vector Backbone Description: Backbone Size:6061; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Flp recombinase-dependent inducible expression vector. DsRed is expressed only in the presence of Flp.
Proper citation: RRID:Addgene_13771 Copy
Vector Backbone Description: Backbone Marker:Anna Behle; Vector Backbone:pSHDY; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32134640
Comments: Please visit https://www.biorxiv.org/content/10.1101/757948v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137661 Copy
Species: Rattus norvegicus
Genetic Insert: GRASP65
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137709 Copy
Species: Homo sapiens
Genetic Insert: Jnk2a1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_13754 Copy
Species: Homo sapiens
Genetic Insert: Jnk1b2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_13752 Copy
Genetic Insert: Hes1 promoter
Vector Backbone Description: Backbone Size:3087; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: The mouse Hes1 promoter is specific for progenitor cells in the retina. This promoter is also weakly active in Muller glia in the differentiated retina.
Proper citation: RRID:Addgene_13767 Copy
Genetic Insert: Nrl promoter
Vector Backbone Description: Backbone Size:3062; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Mouse Nrl promoter was isolated by genomic PCR using B6 mouse genomic DNA as a template.
The Nrl promoter-DsRed is specific for rod photorecetor cells in the retina.
Proper citation: RRID:Addgene_13764 Copy
Species: Homo sapiens
Genetic Insert: Jnk2a2(apf)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8654373
Proper citation: RRID:Addgene_13761 Copy
Species: Herpes simplex virus type I KOS strain
Genetic Insert: HSV-1 glycoprotein E
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:5863; Vector Backbone:pAcUW51; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16646632
Proper citation: RRID:Addgene_13762 Copy
Species: Salmonella enterica serovar Typhimurium
Genetic Insert: SopF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5847; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137734 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeTadA1.0-T7 RNAP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:ColE1/AMP; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707425
Comments: Depositor confirms E366G does not affect plasmid function.
Proper citation: RRID:Addgene_137735 Copy
Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Comments: 3' codons have been altered from original reference sequence, while preserving the amino acid sequence.
Proper citation: RRID:Addgene_137757 Copy
Species: Homo sapiens
Genetic Insert: GABARAP
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137760 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137763 Copy
Genetic Insert: Rhodopsin promoter-Cre
Vector Backbone Description: Backbone Size:5362; Vector Backbone:pCAGEN?; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: The rhodopsin promoter-Cre is specific for photorecetor cells in the retina.
Cre has a Myc-tag at its C-terminus.
Proper citation: RRID:Addgene_13779 Copy
Species: Homo sapiens
Genetic Insert: CD44s (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Comments: Addgene NGS result found mutations I186T and I253V when compared to CD44 [XP_016874074.1]. Depositor confirmed that this does not affect the function of the plasmid.
Proper citation: RRID:Addgene_137812 Copy
Species: Homo sapiens
Genetic Insert: mir30 cassette
Vector Backbone Description: Backbone Size:5555; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).
Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.
Proper citation: RRID:Addgene_13786 Copy
Species: Homo sapiens
Genetic Insert: CD44v8-10 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137823 Copy
Species: Rattus norvegicus
Genetic Insert: Rat beta-arrestin2
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137794 Copy
Species: Homo sapiens
Genetic Insert: FKBP-NTEVp(WT)
Vector Backbone Description: Backbone Size:4321; Vector Backbone:pcDNA3.1/HygroR(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33526893
Comments: Please visit https://www.biorxiv.org/content/10.1101/863530v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_137831 Copy
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