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Species: Synthetic
Genetic Insert: mts
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98818 Copy
Species: Synthetic
Genetic Insert: Codon optimized Cas9
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pJSK316-GPD; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28652865
Proper citation: RRID:Addgene_98906 Copy
Species: Synthetic
Genetic Insert: human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671680
Proper citation: RRID:Addgene_98996 Copy
Species: Synthetic
Genetic Insert: PROPS
Vector Backbone Description: Backbone Marker:Bio Bricks - iGem parts combined by Ben Dodd; Backbone Size:2384; Vector Backbone:BB118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28808010
Proper citation: RRID:Addgene_98919 Copy
Species: Synthetic
Genetic Insert: Venus KRasCT
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29937389
Comments: The Venus KRasCT fragment was made as a gBlock with HindIII and XbaI, cut and cloned into pcDNA3.1
Proper citation: RRID:Addgene_99016 Copy
Species: Synthetic
Genetic Insert: Lamp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_98882 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931898
Proper citation: RRID:Addgene_98886 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-2A-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931898
Proper citation: RRID:Addgene_98885 Copy
Species: Synthetic
Genetic Insert: GCaMP6f tethered to mRuby
Vector Backbone Description: Backbone Marker:Bio Bricks - iGem parts combined by Ben Dodd; Backbone Size:2384; Vector Backbone:BB118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28808010
Proper citation: RRID:Addgene_98920 Copy
Species: Synthetic
Genetic Insert: Ruby2-smFP-Flag
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Comments: Penn Vector Core PV3509
Proper citation: RRID:Addgene_98925 Copy
Species: Synthetic
Genetic Insert: GFP-smFP-myc
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Comments: Penn Vector Core PV3661
Proper citation: RRID:Addgene_98927 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2723. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98929 Copy
Species: Synthetic
Genetic Insert: Neomycin-T2A-ISce1-ISce1-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:6700; Vector Backbone:pLenti CMV Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959974
Proper citation: RRID:Addgene_98895 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2914. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98930 Copy
Species: Synthetic
Genetic Insert: Neomycin-T2A-mCherry-GFP
Vector Backbone Description: Backbone Size:3050; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959974
Proper citation: RRID:Addgene_98896 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2724. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98931 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Mathupala, S.P.; Backbone Size:6059; Vector Backbone:pLXIN2; Vector Types:Mammalian Expression, Retroviral, Bicistronic retroviral expression vector; Bacterial Resistance:Ampicillin
Comments: When transfected into amphotropic (PA317) or ecotropic (PE501) mammalian packaging cell lines, the plasmid pLXIN2 will generate live retrovirus. Thus, the procedures will require handling at BSL2 level, when the plasmid is used to generate recombinant retrovirus for transduction.
Please note the Kozak sequence is missing a nucleotide; however, this has no functional consequence for expression.
Proper citation: RRID:Addgene_99204 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Dr. Dusty Miller, Fred Hutchinson Cancer Center, Seattle, WA; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: When transfected into packaging cell lines PE501 (ecotropic) or PA317 (amphotropic) the plasmid will generate live retrovirus. Thus, under these conditions BSL2 safety will need to be observed.
Proper citation: RRID:Addgene_99207 Copy
Species: Synthetic
Genetic Insert: 47xCAG 12xMS2 hairpins
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8700; Vector Backbone:pHR-TRE3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Proper citation: RRID:Addgene_99148 Copy
Species: Synthetic
Genetic Insert: 29xGGGGCC 12xMS2 hairpins
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8700; Vector Backbone:pHR-TRE3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Comments: 29x GGGGCC cannot be confirmed by sequencing due to high GC content. Addgene was able to verify at least 16x repeats. Instability may be observed in the repeat region so it is recommended that multiple DNA preparations be screened.
Proper citation: RRID:Addgene_99149 Copy
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