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Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35610221
Comments: PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2-TRPL25(Arm 3)- ARS305-PTEF1> yEGFP> TURA3
Proper citation: RRID:Addgene_185871 Copy
Species: Homo sapiens
Genetic Insert: INSR
Vector Backbone Description: Vector Backbone:MAC-tag-C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_187772 Copy
Species: M13 bacteriophage
Genetic Insert: M13 gene III
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pLITMUS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27982027
Comments: Addgene QC NGS identifies a SNP at nucleotide 1471 in the M13 ori. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_80908 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15223344
Proper citation: RRID:Addgene_186706 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27980067
Proper citation: RRID:Addgene_186705 Copy
Species: Homo sapiens
Genetic Insert: PKMYT1-V5-TurboID
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135247 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:modRNA cap0 plasmid; Vector Types:IVT template plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36160051
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170180 Copy
Species: Synthetic
Genetic Insert: Electra2-P2A-EGFP
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pAAV-CAG-EGFP-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184936 Copy
Species: Synthetic
Genetic Insert: ALD(-125, 40)- ALD6(1054, 1749)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34499421
Proper citation: RRID:Addgene_185866 Copy
Species: Synthetic
Genetic Insert: EBFP2-P2A-EGFP
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pAAV-CAG-EGFP-WPRE; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184938 Copy
Species: Synthetic
Genetic Insert: mClover3
Vector Backbone Description: Vector Backbone:pGRG36; Vector Types:Bacterial Expression, miniTn7 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118555 Copy
Species: Homo sapiens
Genetic Insert: 1G4 TCR alpha
Vector Backbone Description: Vector Backbone:pHRSin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187572 Copy
Species: Synthetic
Genetic Insert: PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173283
Proper citation: RRID:Addgene_185840 Copy
Species: Other
Genetic Insert: Cas9-2A-Puro
Vector Backbone Description: Vector Backbone:modRNA cap0 plasmid; Vector Types:IVT template plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36160051
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172855 Copy
Species: Aedes aegypti
Genetic Insert: AAEL003877-dCAS9-VPR
Vector Backbone Description: Backbone Size:5246; Vector Backbone:Plasmid #100581; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36656895
Proper citation: RRID:Addgene_183993 Copy
Species: Synthetic
Genetic Insert: see comments
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35610221
Comments: PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PGAL1>ERG20F96C>TEBS1-PSk.GAL2>Xd.CRtYBE83K>TCYC1-PSe.GAL2>XdCrtI>TRPL41B-PBTS1> RPL25(partial; Arm2)
Proper citation: RRID:Addgene_185890 Copy
Species: Homo sapiens
Genetic Insert: dimeric MBD1
Vector Backbone Description: Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34586782
Proper citation: RRID:Addgene_186462 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::Cas9, Cbr-unc-119
Vector Backbone Description: Vector Backbone:pMLS621; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188892 Copy
Species: Homo sapiens
Genetic Insert: GPa3b17 hTCR alpha
Vector Backbone Description: Vector Backbone:pHRi; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35985289
Proper citation: RRID:Addgene_187601 Copy
Species: Oryza sativa
Genetic Insert: OsCYP711A2
Vector Backbone Description: Backbone Size:6544; Vector Backbone:pAG416-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178301 Copy
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