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Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35078 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35077 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35076 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35075 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35081 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35080 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35068 Copy
Species: Homo sapiens
Genetic Insert: IFI16-(192-393)-HinA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397192
Comments: Growth conditions for protein production: BL21DE3-Codon-plus-RIL
Proper citation: RRID:Addgene_35060 Copy
Species: Homo sapiens
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15571721
Comments: Protein expression in BL21DE3-Gold. Induce culture at OD600=0.8 with 0.5mM IPTG, 37oC for 3 hours.
Proper citation: RRID:Addgene_35066 Copy
Species: Homo sapiens
Genetic Insert: IFI16-(192-393)-HinA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397192
Comments: Growth conditions for protein production: BL21DE3-Codon-plus-RIL
Proper citation: RRID:Addgene_35058 Copy
Species: Homo sapiens
Genetic Insert: IFI16-(192-393)-HinA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397192
Comments: Growth conditions for protein production: BL21DE3-Codon-plus-RIL
Proper citation: RRID:Addgene_35057 Copy
Species: Homo sapiens
Genetic Insert: IFI16-(192-393)-HinA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397192
Comments: Growth conditions for protein production: BL21DE3-Codon-plus-RIL
Proper citation: RRID:Addgene_35056 Copy
Species: Homo sapiens
Genetic Insert: IFI16-(192-393)-HinA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397192
Comments: Growth conditions for protein production: BL21DE3-Codon-plus-RIL
Proper citation: RRID:Addgene_35055 Copy
Species: Homo sapiens
Genetic Insert: Zer1
Vector Backbone Description: Vector Backbone:MSCV-IP N-V5; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35100 Copy
Species: Homo sapiens
Genetic Insert: Protein Kinase C iota
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4
Proper citation: RRID:Addgene_35387 Copy
Species: Homo sapiens
Genetic Insert: hPLK2 K111M
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2K111M and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35386 Copy
Species: Homo sapiens
Genetic Insert: hPLK2
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2WT and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35385 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:5609; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human elongation factor-1a promoter. Golgi export trafficking signal (TS) sequence is KSRITSEGEYIPLDQIDINV. ER export sequence is FCYENEV.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26966 Copy
Species: Homo sapiens
Genetic Insert: hChR2(E123T/H134R)-EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5587; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20081849
Comments: This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26968 Copy
Species: Homo sapiens
Genetic Insert: shRNA NEDD4L #1
Vector Backbone Description: Backbone Size:6401; Vector Backbone:pRetroSuper; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917253
Comments: shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa
Proper citation: RRID:Addgene_27016 Copy
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