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Species: Human papillomavirus type 2a
Genetic Insert: HPV2a E7
Vector Backbone Description: Backbone Size:8700; Vector Backbone:MSCV-P C-Flag-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35137 Copy
Species: Human papillomavirus type 45
Genetic Insert: HPV45 E7
Vector Backbone Description: Vector Backbone:MSCV-P C-Flag-HA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35135 Copy
Species: HPV18
Genetic Insert: HPV18 E2
Vector Backbone Description: Vector Backbone:MSCV-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35134 Copy
Species: E.coli
Genetic Insert: kanMX4
Vector Backbone Description: Vector Backbone:pXP45; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16462944
Proper citation: RRID:Addgene_35133 Copy
Species: C.albicans
Genetic Insert: fca1MX3
Vector Backbone Description: Vector Backbone:pFA6-kanMX3; Vector Types:Yeast Replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16088873
Proper citation: RRID:Addgene_35098 Copy
Species: Bacteria
Genetic Insert: hygMX4/T-urf13
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast Replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16462944
Proper citation: RRID:Addgene_35131 Copy
Species: Streptomyces noursei
Genetic Insert: natMX4
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16462944
Proper citation: RRID:Addgene_35130 Copy
Species: Streptomyces noursei
Genetic Insert: nat1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35126 Copy
Species: Streptomyces viridochromogenes
Genetic Insert: patMX3
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35124 Copy
Species: Candida albicans
Genetic Insert: URA3
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35129 Copy
Species: Klebsiella pneumoniae
Genetic Insert: hphMX4
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35122 Copy
Species: Streptomyces noursei
Genetic Insert: natMX4
Vector Backbone Description: Vector Backbone:pFA6; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10514571
Proper citation: RRID:Addgene_35121 Copy
Species: C.albicans
Genetic Insert: callys5MX3
Vector Backbone Description: Vector Backbone:pFA6-MX4; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35113 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: lys5MX3
Vector Backbone Description: Vector Backbone:pFA6-kanMX4; Vector Types:Yeast Replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35112 Copy
Species: C.albicans
Genetic Insert: callys5MX4
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: lys5MX4
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14745782
Proper citation: RRID:Addgene_35116 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:10033; Vector Backbone:pBID-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35195 Copy
Species: Synthetic
Genetic Insert: PCP-2x-yeGFP
Vector Backbone Description: Vector Backbone:pURA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21512033
Comments: This plasmid expresses a chimeric RNA-binding protein fused to GFP used to detect mRNAs that contain multiple copies of an RNA sequence (see Addgene plasmid #'s 35191, 35192 & 35196). See associated publication for detailed methods and use of this plasmid.
Please note that Addgene's sequencing results identified discrepancies when compared to the assembled sequence provided by the depositing laboratory. The first GFP construct in this plasmid (bp#441-1154) is yeast codon optimized and contains a E124K mutation and amino acid 206 is A (not K, as suggested by the depositor's sequence). The second GFP construct in this plasmid (bp# 1212-1928) is NOT yeast codon optimized.
This construct has successfully been used by the depositing laboratory as detailed in the associated publication and the sequencing discrepancies are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_35194 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:13081; Vector Backbone:pBID-UAS-GGi; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven RNA inhibition (RNAi). 2 inverted gateway cloning cassettes are flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, hsp70 basal promoter. ftz intron facilitates RNA hairpin formation and expression. Transgene selection using white gene. loxP site facilitates elimination of
transgene markers via Cre recombinasemediated
excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please note that the inverted sequences present can cause rearrangements of this vector. Recommend miniprep only and confirm plasmid map subsequent to amplification or cloning. After gateway recombination, recommend amplification in Stbl2 cells [Invitrogen Cat. 10268-019] and combining minipreps for Drosophila injection.
Addgene's sequencing results indicated a single nucleotide mismatch at bp# 5286 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect plasmid function.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35199 Copy
Species: Synthetic
Genetic Insert: GLT1 POL1 24xPP7SL
Vector Backbone Description: Vector Backbone:pHIS; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21512033
Comments: Note that Addgene NGS results found 22xPP7 stem loop repeats rather than expected 24. It is not known how this impacts plasmid function.
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.
Proper citation: RRID:Addgene_35196 Copy
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