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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 3987-4020
Proper citation: RRID:Addgene_48975 Copy
Vector Backbone Description: Backbone Marker:Malcolm Moore lab; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: AmCyan1 excitation and emission maxima are 458 and 489 nm, respectively
Proper citation: RRID:Addgene_48974 Copy
Species: Synthetic
Genetic Insert: 3xFNLDD
Vector Backbone Description: Backbone Marker:Toshio Kitamura; Backbone Size:5994; Vector Backbone:pMXs-IG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25607658
Proper citation: RRID:Addgene_48972 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4014-4047
Proper citation: RRID:Addgene_48976 Copy
Species: Homo sapiens
Genetic Insert: MALT1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22309193
Proper citation: RRID:Addgene_48971 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48964 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48963 Copy
Vector Backbone Description: Backbone Marker:life technologies; Vector Backbone:pMA-T; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Proper citation: RRID:Addgene_48961 Copy
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_49016 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Comments: Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
Proper citation: RRID:Addgene_48966 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48965 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:life technologies; Backbone Size:4959; Vector Backbone:pDEST; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_48960 Copy
Species: Homo sapiens
Genetic Insert: dCas9_VP64 (human-codon-optimized)
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Comments: L591M mutation with dCas9
Proper citation: RRID:Addgene_49015 Copy
Species: Homo sapiens
Genetic Insert: Malic Enzyme 1
Vector Backbone Description: Backbone Marker:Addgene plasmid # 1764; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23334421
Proper citation: RRID:Addgene_49163 Copy
Species: Enterobacteria phage T4
Genetic Insert: Bacteriophage T4 gene 600
Vector Backbone Description: Backbone Size:2662; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23492219
Proper citation: RRID:Addgene_49159 Copy
Species: Synthetic
Genetic Insert: ssAAV-smCBA-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49142 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple155-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531684
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Additional References:
1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019).
2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016).
3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015).
4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
Proper citation: RRID:Addgene_49140 Copy
Species: Synthetic
Genetic Insert: ssAAV-Ple67-emGFP-WPRE
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30765420
Comments: Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49138 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Backbone Size:6833; Vector Backbone:pEF5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23930224
Proper citation: RRID:Addgene_49099 Copy
Species: Synthetic
Genetic Insert: ssAAV-hs1218-iCre
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30062914
Comments: Additional reference for hs1218 promoter: Visel et al., 2007. VISTA Enhancer Browser--a database of tissue-specific human enhancers. Nucleic Acids Res. 2007 Jan;35(Database issue):D88-92. Epub 2006 Nov 27. PMID: 17130149 (https://www.ncbi.nlm.nih.gov/pubmed/17130149)
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Proper citation: RRID:Addgene_49130 Copy
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