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Species: Physeter macrocephalus (Sperm Whale)
Genetic Insert: Sperm Whale Myoglobin
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3321062
Proper citation: RRID:Addgene_20058 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20051 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:PSB819; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043403
Proper citation: RRID:Addgene_20030 Copy
Species: S. lycopersicum
Genetic Insert: 2A11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONRP4-P1R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20089 Copy
Species: Homo sapiens
Genetic Insert: transcription elongation factor B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5022; Vector Backbone:pcDNA3.1(+)-Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:10878807
Proper citation: RRID:Addgene_20000 Copy
Species: S. lycopersicum
Genetic Insert: PHD
Vector Backbone Description: Backbone Size:4777; Vector Backbone:pDONR221-P4P1R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Comments: Depositing scientist is aware of discrepancies between Addgene's sequence and their own. They have confirmed the differences and tested the plasmids--they are completely functional.
Proper citation: RRID:Addgene_20088 Copy
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:0; Vector Backbone:pESC-leu2; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16612385
Proper citation: RRID:Addgene_20120 Copy
Species: S. lycopersicum
Genetic Insert: PNH promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONRP4-P1R; Vector Types:Synthetic Biology, plant Gateway Donor vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20083 Copy
Species: Mus musculus
Genetic Insert: Nfkb2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20019 Copy
Species: Mus musculus
Genetic Insert: RelB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20017 Copy
Species: Mus musculus
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166378
Comments: See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20012 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16166378
Comments: This pcDNA3 plasmid contains a new poly-linker and single c-terminal flag-tag sequence for the expression of NF-kBs in mammalian cell lines. See map for more information.
Proper citation: RRID:Addgene_20011 Copy
Genetic Insert: DsRed
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pDONR221-P4R-P3R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20099 Copy
Genetic Insert: GFPGUS
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pDONR221-P4R-P3R; Vector Types:Synthetic Biology, plant Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20098 Copy
Species: CaMV
Genetic Insert: 2x35S
Vector Backbone Description: Backbone Size:4777; Vector Backbone:pDONR221-P4P1R; Vector Types:Synthetic Biology, Gateway vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_20093 Copy
Vector Backbone Description: Backbone Size:4525; Vector Backbone:p1NIL; Vector Types:Suicide vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20187 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18788807
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of FKBP
Proper citation: RRID:Addgene_20220 Copy
Species: Mus musculus
Genetic Insert: Notch 3 Intracellular Domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3
(5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
Proper citation: RRID:Addgene_20185 Copy
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pBR322; Vector Types:Marker cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10931901
Proper citation: RRID:Addgene_20189 Copy
Species: Mus musculus
Genetic Insert: Notch 2 intracellular domain
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365048
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD2 was cloned into XbaI/BamHI sites. NICD2 (Met-1699 to Ala-2470 of full-length mouse N2) was amplified from the N2 plasmid (gift from Dr. Y. Hamada) using primers 5XbaN2 (5'-GCTCTAGAATGGCCAAGCGGAAGCGCAAG-3') and 3BamN2
(5'-GCGGATCCCTATGCATACACCTGCATGTTGCTG-3').
Please note that Addgene sequencing results confirm the updated domain numbering to be Met 1701-Ala 2473.
Proper citation: RRID:Addgene_20184 Copy
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