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Species: Synthetic
Genetic Insert: human codon-optimized Vika sequence, NLS tagged
Vector Backbone Description: Vector Backbone:pNPK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23143104
Proper citation: RRID:Addgene_79969 Copy
Species: Synthetic
Genetic Insert: EPN-01*
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Comments: EPN-01* contains an M at position 20, which functions as an internal start codon and appeared as a double band in western blots. EPN-01 (without the *) contain a M20I mutation when compared to EPN-01*
Proper citation: RRID:Addgene_80039 Copy
Species: Synthetic
Genetic Insert: I3-01-Myc
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80038 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80036 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80035 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80034 Copy
Species: Synthetic
Genetic Insert: EPN-01(deltaYP)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80050 Copy
Species: Synthetic
Genetic Insert: miRFP709
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3999; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79986 Copy
Species: Synthetic
Genetic Insert: miRFP703
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3999; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79985 Copy
Species: Synthetic
Genetic Insert: miRFP670
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79987 Copy
Species: Synthetic
Genetic Insert: miRFP709
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4012; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27539380
Proper citation: RRID:Addgene_79989 Copy
Species: Synthetic
Genetic Insert: pCMV-EPN-01*(ΔL2)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80042 Copy
Species: Synthetic
Genetic Insert: EPN-01(L33R)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Comments: Please note that the L33R mutation is numbered according to the original I3-01 protein and is L50R when numbered according to EPN-01.
Proper citation: RRID:Addgene_80048 Copy
Species: Synthetic
Genetic Insert: EPN-01
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80046 Copy
Species: Synthetic
Genetic Insert: EPN-01*(L33R)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80044 Copy
Species: Synthetic
Genetic Insert: eGFP(1-155)
Vector Backbone Description: Backbone Marker:Kodama and Wada 2009; Backbone Size:3459; Vector Backbone:pBRN169-MXMT; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80130 Copy
Species: Synthetic
Genetic Insert: dGBP1-TagBFP
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27205882
Comments: Please cite: Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis EG, Li JZ, Cepko CL (2016) Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife 5. doi:10.7554/eLife.15312
Proper citation: RRID:Addgene_80086 Copy
Species: Synthetic
Genetic Insert: Flp-DOG
Vector Backbone Description: Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27205882
Comments: Please cite: Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis EG, Li JZ, Cepko CL (2016) Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife 5. doi:10.7554/eLife.15312
Proper citation: RRID:Addgene_80084 Copy
Species: Synthetic
Genetic Insert: psGFP(1-155)
Vector Backbone Description: Backbone Marker:Kodama and Wada 2009; Backbone Size:3459; Vector Backbone:pBRN169-MXMT; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80170 Copy
Species: Synthetic
Genetic Insert: frGFP(1-155)
Vector Backbone Description: Backbone Marker:Kodama and Wada 2009; Backbone Size:3459; Vector Backbone:pBRN169-MXMT; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80164 Copy
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