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Vector Backbone Description: Backbone Marker:NA, NA, Invitrogen; Vector Backbone:pGP704, pBAD33, pDONR201; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34801582
Comments: The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208).
Proper citation: RRID:Addgene_178083 Copy
Species: Escherichia coli
Genetic Insert: TcR (tetA/tetR)
Vector Backbone Description: Backbone Size:2677; Vector Backbone:pMK2010; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34801582
Comments: The plasmids from this article are also used with plasmid pXint129 (www.addgene.org/178208).
Proper citation: RRID:Addgene_178086 Copy
Species: A. victoria
Genetic Insert: GFPuv
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16333863
Comments: Provided in strain JSB, a slightly modified version of E. coli strain BL21 with genotype: BL21(DE3)/D(sbm-ygfDGHI).
Proper citation: RRID:Addgene_17813 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178034 Copy
Species: Mus musculus
Genetic Insert: Cdk2ap1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression, Nonviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34644528
Proper citation: RRID:Addgene_178032 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178141 Copy
Vector Backbone Description: Backbone Size:5135; Vector Backbone:pPro18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269719
Comments: The PCR product containing prpR and PprpB was digested with ClaI and NheI and ligated to the large fragments of pBAD18 resulting from digestion with the same enzymes, creating pPro18.
Please refer to the Addgene verified sequence for the correct orientation of the MCS.
Proper citation: RRID:Addgene_17806 Copy
Vector Backbone Description: Backbone Size:5445; Vector Backbone:pPro30; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16269719
Comments: After deletion of the NheI site in the front of pACYC origin region of pBAD30 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD30 resulting from digestion with the same enzymes, creating pPro30.
Proper citation: RRID:Addgene_17809 Copy
Species: Synthetic
Genetic Insert: Lrig1 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178048 Copy
Species: Synthetic
Genetic Insert: Renilla_713 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178046 Copy
Species: Synthetic
Genetic Insert: Pten_1523 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178044 Copy
Species: Synthetic
Genetic Insert: Luciferase_1309 shRNAmir
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, RNAi, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: mir-30a "miR-E" shRNAmir expression cassette sequence was obtained from Fellmann et al., 2013 (https://doi.org/10.1016/j.celrep.2013.11.020) and synthesized.
Proper citation: RRID:Addgene_178045 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178235 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178234 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178239 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178237 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178238 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178230 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178224 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178225 Copy
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