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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3327; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000037; Origin: p15A
Proper citation: RRID:Addgene_35271 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3281; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000036; Origin: colE1
Proper citation: RRID:Addgene_35270 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4749; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000040; Origin: SC101
Proper citation: RRID:Addgene_35274 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3126; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000035; Origin: colE1
Proper citation: RRID:Addgene_35269 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3340; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000034; Origin: colE1
Proper citation: RRID:Addgene_35268 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:5360; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000067; Origin: SC101
Proper citation: RRID:Addgene_35301 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3957; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000072; Origin: colE1
Proper citation: RRID:Addgene_35306 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:5301; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000069; Origin: SC101
Proper citation: RRID:Addgene_35303 Copy
Species: Tarsius bancanus
Genetic Insert: MER20/MER39
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22733751
Proper citation: RRID:Addgene_35261 Copy
Species: Mus musculus
Genetic Insert: MER77
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_35262 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3789; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000074; Origin: p15A
Proper citation: RRID:Addgene_35308 Copy
Species: Colobus guereza
Genetic Insert: MER20/MER39
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22733751
Proper citation: RRID:Addgene_35258 Copy
Species: Pongo pygmaeus
Genetic Insert: MER20/MER39
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22733751
Proper citation: RRID:Addgene_35257 Copy
Species: Loxodonta africana
Genetic Insert: dPRL promoter for elephant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21813467
Proper citation: RRID:Addgene_35255 Copy
Vector Backbone Description: Backbone Size:9506; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35250 Copy
Vector Backbone Description: Backbone Size:7773; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35253 Copy
Vector Backbone Description: Backbone Size:9298; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:22211571
Comments: MIGS vectors are based on pGREEN series:
Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.
http://www.pgreen.ac.uk/
obtained from John Innes Centre, UK
Proper citation: RRID:Addgene_35247 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting si:ch211-221n23.1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene si:ch211-221n23.1. Please note that this plasmid does NOT contain the si:ch211-221n23.1 sequence.
Users must order the complementary plasmid si:ch211-221n23.1_L (OZ603) [Addgene plasmid 35243] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GCTGCAGAG-3' in a bacterial two hybrid assay, and resulted in 9.9 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_35244 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting si:ch211-221n23.1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene si:ch211-221n23.1. Please note that this plasmid does NOT contain the si:ch211-221n23.1 sequence.
Users must order the complementary plasmid si:ch211-221n23.1_R (OZ604) [Addgene plasmid 35244] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GCAGTTGCG-3' in a bacterial two hybrid assay, and resulted in 9.5 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_35243 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting panx2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene panx2. Please note that this plasmid does NOT contain the panx2 sequence.
Users must order the complementary plasmid panx2_L (OZ601) [Addgene plasmid 35241] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GTGGTAGCA-3' in a bacterial two hybrid assay, and resulted in 15.7 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_35242 Copy
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