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Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145175 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145173 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145171 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145245 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145149 Copy
Species: Homo sapiens
Genetic Insert: Angiotensin-converting enzyme 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145148 Copy
Species: SARS coronavirus 2
Genetic Insert: Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145145 Copy
Species: Homo sapiens
Genetic Insert: immunoglobulin J chain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5332; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32753553
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_145146 Copy
Species: Homo sapiens
Genetic Insert: MED1-IDR long
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145276 Copy
Vector Backbone Description: Vector Backbone:pmCellFree_KA2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32081002
Proper citation: RRID:Addgene_145369 Copy
Species: Homo sapiens
Genetic Insert: sodium voltage-gated channel alpha subunit 5
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pCDNA3.1-Zeo (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15548568
Proper citation: RRID:Addgene_145374 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5C B56 epsilon regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14537 Copy
Species: Mus musculus
Genetic Insert: MKK7a1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14538 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5D B56 delta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function.
Proper citation: RRID:Addgene_14536 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5B B56 beta regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Addgene NGS identified an I346V mutation in the B56β ORF, this is not predicted to impact plasmid function.
Proper citation: RRID:Addgene_14533 Copy
Species: Homo sapiens
Genetic Insert: PPP2R5A B56 alpha regulatory subunit of PP2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10186; Vector Backbone:pCEP4 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10092233
Comments: 4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
Proper citation: RRID:Addgene_14532 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Ala)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14539 Copy
Species: Homo sapiens
Genetic Insert: ATM shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14542 Copy
Species: Homo sapiens
Genetic Insert: ATR shRNA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRDI292; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15709017
Comments: Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_14543 Copy
Species: Mus musculus
Genetic Insert: MKK7a1(Glu)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9207092
Proper citation: RRID:Addgene_14540 Copy
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