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Species: Synthetic
Genetic Insert: iRFP670-tDeg
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34431301
Proper citation: RRID:Addgene_185403 Copy
Species: Synthetic
Genetic Insert: PPGK-driven GS-linker
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169481 Copy
Species: Synthetic
Genetic Insert: PCMV-driven monomeric cyan fluorescent protein mTFP1
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169511 Copy
Species: Synthetic
Genetic Insert: PCMV-driven monomeric cyan fluorescent protein mTurquoise2
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169512 Copy
Species: Synthetic
Genetic Insert: PCMV-driven fluorescent timer, fast maturation time
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169518 Copy
Species: Synthetic
Genetic Insert: PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
Vector Backbone Description: Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169519 Copy
Species: Synthetic
Genetic Insert: tdPCP-GFP
Vector Backbone Description: Backbone Marker:Addgene #54642; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Comments: under control of the weakened deltaTATA-CMV promoter for low level expression
Proper citation: RRID:Addgene_183934 Copy
Species: Synthetic
Genetic Insert: tdMCP-tdTomato
Vector Backbone Description: Backbone Marker:Addgene #54642; Backbone Size:5443; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Comments: under control of the weakened deltaTATA-CMV promoter for low level expression
Proper citation: RRID:Addgene_183938 Copy
Species: Synthetic
Genetic Insert: tdPCP-GFP-VPR
Vector Backbone Description: Backbone Marker:Addgene #54642; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Comments: under control of the weakened deltaTATA-CMV promoter for low level expression
Proper citation: RRID:Addgene_183936 Copy
Species: Synthetic
Genetic Insert: MTRIA-5HT
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184597 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-MaCPNS1 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35643078
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336 . The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_185136 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-MaCPNS2 VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35643078
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336 . The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_185137 Copy
Species: Synthetic
Genetic Insert: MTRIA-CCK
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184602 Copy
Species: Synthetic
Genetic Insert: rTetR-GFP-VPR
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183918 Copy
Species: Synthetic
Genetic Insert: rTetR-GFP-VP16
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183917 Copy
Species: Synthetic
Genetic Insert: tdTomato-LacI
Vector Backbone Description: Backbone Marker:Addgene #54642; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183921 Copy
Species: Synthetic
Genetic Insert: SNAPtag-LacI
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183922 Copy
Species: Synthetic
Genetic Insert: MTRIA-NPY
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184613 Copy
Species: Synthetic
Genetic Insert: MTRIA-NMB
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184610 Copy
Species: Synthetic
Genetic Insert: MTRIA-NOC
Vector Backbone Description: Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36138174
Comments: Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
Proper citation: RRID:Addgene_184611 Copy
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