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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pBbA8a-RFP Resource Report Resource Website |
RRID:Addgene_35271 | RFP | Synthetic | Ampicillin | PMID:21933410 | JBEI Part ID: JPUB_000037; Origin: p15A | Backbone Size:3327; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 0 | |
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pBbE8k-RFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_35270 | RFP | Synthetic | Kanamycin | PMID:21933410 | JBEI Part ID: JPUB_000036; Origin: colE1 | Backbone Size:3281; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:59 | 5 | |
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pBbS8a-RFP Resource Report Resource Website |
RRID:Addgene_35274 | RFP | Synthetic | Ampicillin | PMID:21933410 | JBEI Part ID: JPUB_000040; Origin: SC101 | Backbone Size:4749; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 0 | |
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pBbE8c-RFP Resource Report Resource Website |
RRID:Addgene_35269 | RFP | Synthetic | Chloramphenicol | PMID:21933410 | JBEI Part ID: JPUB_000035; Origin: colE1 | Backbone Size:3126; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:59 | 0 | |
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pBbE8a-RFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_35268 | RFP | Synthetic | Ampicillin | PMID:21933410 | JBEI Part ID: JPUB_000034; Origin: colE1 | Backbone Size:3340; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 1 | |
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pBbS4a-RFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_35301 | RFP | Synthetic | Ampicillin | PMID:21933410 | JBEI Part ID: JPUB_000067; Origin: SC101 | Backbone Size:5360; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 1 | |
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pBbE3k-RFP Resource Report Resource Website |
RRID:Addgene_35306 | RFP | Synthetic | Kanamycin | PMID:21933410 | JBEI Part ID: JPUB_000072; Origin: colE1 | Backbone Size:3957; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:59 | 0 | |
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pBbS4k-RFP Resource Report Resource Website |
RRID:Addgene_35303 | RFP | Synthetic | Kanamycin | PMID:21933410 | JBEI Part ID: JPUB_000069; Origin: SC101 | Backbone Size:5301; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:59 | 0 | |
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TarsierMER20MER39 Resource Report Resource Website |
RRID:Addgene_35261 | MER20/MER39 | Tarsius bancanus | Ampicillin | PMID:22733751 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 0 | ||
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mouseMER77 Resource Report Resource Website |
RRID:Addgene_35262 | MER77 | Mus musculus | Ampicillin | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:59 | 0 | |||
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pBbA3c-RFP Resource Report Resource Website |
RRID:Addgene_35308 | RFP | Synthetic | Chloramphenicol | PMID:21933410 | JBEI Part ID: JPUB_000074; Origin: p15A | Backbone Size:3789; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:13:59 | 0 | |
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colobusMER20MER39 Resource Report Resource Website |
RRID:Addgene_35258 | MER20/MER39 | Colobus guereza | Ampicillin | PMID:22733751 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 0 | ||
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orangutanMER20MER39 Resource Report Resource Website |
RRID:Addgene_35257 | MER20/MER39 | Pongo pygmaeus | Ampicillin | PMID:22733751 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:59 | 0 | ||
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elephant.dPRL Resource Report Resource Website |
RRID:Addgene_35255 | dPRL promoter for elephant | Loxodonta africana | Ampicillin | PMID:21813467 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:PGL4.70; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:05 | 0 | ||
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MIGS3.2 (FF574) Resource Report Resource Website |
RRID:Addgene_35250 | Chloramphenicol and Spectinomycin | PMID:22211571 | MIGS vectors are based on pGREEN series: Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol., 42, 819-832. http://www.pgreen.ac.uk/ obtained from John Innes Centre, UK | Backbone Size:9506; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:13:59 | 0 | |||
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MIGS5.1 (FF572) Resource Report Resource Website |
RRID:Addgene_35253 | Chloramphenicol and Spectinomycin | PMID:22211571 | MIGS vectors are based on pGREEN series: Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol., 42, 819-832. http://www.pgreen.ac.uk/ obtained from John Innes Centre, UK | Backbone Size:7773; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:13:59 | 0 | |||
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MIGS2.1 (FF570) Resource Report Resource Website |
RRID:Addgene_35247 | Chloramphenicol and Spectinomycin | PMID:22211571 | MIGS vectors are based on pGREEN series: Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol., 42, 819-832. http://www.pgreen.ac.uk/ obtained from John Innes Centre, UK | Backbone Size:9298; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin | 2026-08-15 01:13:59 | 0 | |||
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si:ch211-221n23.1_R (OZ604) Resource Report Resource Website |
RRID:Addgene_35244 | Zinc finger array targeting si:ch211-221n23.1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene si:ch211-221n23.1. Please note that this plasmid does NOT contain the si:ch211-221n23.1 sequence. Users must order the complementary plasmid si:ch211-221n23.1_L (OZ603) [Addgene plasmid 35243] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGCAGAG-3' in a bacterial two hybrid assay, and resulted in 9.9 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:59 | 0 | |
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si:ch211-221n23.1_L (OZ603) Resource Report Resource Website |
RRID:Addgene_35243 | Zinc finger array targeting si:ch211-221n23.1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene si:ch211-221n23.1. Please note that this plasmid does NOT contain the si:ch211-221n23.1 sequence. Users must order the complementary plasmid si:ch211-221n23.1_R (OZ604) [Addgene plasmid 35244] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCAGTTGCG-3' in a bacterial two hybrid assay, and resulted in 9.5 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:05 | 0 | |
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panx2_R (OZ602) Resource Report Resource Website |
RRID:Addgene_35242 | Zinc finger array targeting panx2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene panx2. Please note that this plasmid does NOT contain the panx2 sequence. Users must order the complementary plasmid panx2_L (OZ601) [Addgene plasmid 35241] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GTGGTAGCA-3' in a bacterial two hybrid assay, and resulted in 15.7 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:59 | 0 |
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