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Species: Synthetic
Genetic Insert: roGFP2-Tsa2dCpdCr
Vector Backbone Description: Vector Backbone:p415-TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089028
Proper citation: RRID:Addgene_83239 Copy
Species: Synthetic
Genetic Insert: roGFP2-Tsa2dCr
Vector Backbone Description: Vector Backbone:p415-TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089028
Proper citation: RRID:Addgene_83238 Copy
Species: Synthetic
Genetic Insert: dCas9-AID P182X
Vector Backbone Description: Vector Backbone:M091; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27723754
Proper citation: RRID:Addgene_83260 Copy
Species: Synthetic
Genetic Insert: Bidirectional CAG Promoter
Vector Backbone Description: Backbone Size:1930; Vector Backbone:modified MXS_chaining vector Addgene 62394; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27152616
Comments: Due to the repeated CAG promoter sequences, a SpeI + EcoRI digest is recommended to confirm the integrity of the chicken-actin/beta globin promoter fragments. Please see link to a diagnostic digest image in the Resource Information section above.
Proper citation: RRID:Addgene_78175 Copy
Species: Synthetic
Genetic Insert: Sacsin Mustela putorius furo 94-1381
Vector Backbone Description: Backbone Marker:Takara (Japan); Backbone Size:4392; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78116 Copy
Species: Synthetic
Genetic Insert: Sacsin Leptonychotes weddellii 89-1367
Vector Backbone Description: Backbone Marker:Takara (Japan); Backbone Size:4392; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78115 Copy
Species: Synthetic
Genetic Insert: erGAP3
Vector Backbone Description: Backbone Size:5397; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27291400
Proper citation: RRID:Addgene_78118 Copy
Species: Synthetic
Genetic Insert: Sacsin Phoenix dactylifera 1-1295
Vector Backbone Description: Backbone Marker:Takara (Japan); Backbone Size:4392; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78117 Copy
Species: Synthetic
Genetic Insert: Sacsin Chlorocebus sabaeus 86-1375
Vector Backbone Description: Backbone Marker:Takara (Japan); Backbone Size:4392; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78112 Copy
Species: Synthetic
Genetic Insert: Sacsin Ceratotherium-simum-simum 89-1366
Vector Backbone Description: Backbone Marker:Takara (Japan); Backbone Size:4392; Vector Backbone:pColdII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78111 Copy
Species: Synthetic
Genetic Insert: erGAP2
Vector Backbone Description: Backbone Size:5397; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_78120 Copy
Species: Synthetic
Genetic Insert: Murine leukemia reverse transcriptase
Vector Backbone Description: Backbone Size:3514; Vector Backbone:pVRb; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27091073
Proper citation: RRID:Addgene_78234 Copy
Species: Synthetic
Genetic Insert: mutated miR-34 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Proper citation: RRID:Addgene_78259 Copy
Species: Synthetic
Genetic Insert: fully complimentary miR-34 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Comments: Please note there is a single nucleotide mismatch between the published miR34 sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function.
Proper citation: RRID:Addgene_78258 Copy
Species: Synthetic
Genetic Insert: mutated let-7 target site
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase, microRNA activity; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26996824
Proper citation: RRID:Addgene_78261 Copy
Species: Synthetic
Genetic Insert: Lyn Fak biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21792185
Comments: To observe the FAK activity at DRM regions, Lyn–FAK biosensor was constructed by fusing a DRM-targeting motif containing myristoylation and palmitoylation sites (glycine and cysteine) derived from Lyn, at the N-terminus of the cytosolic FAK (Cyto-FAK) biosensor (Addgene plasmid 78300).
Proper citation: RRID:Addgene_78299 Copy
Species: Synthetic
Genetic Insert: TurboFP635
Vector Backbone Description: Backbone Marker:Seeliger et al., 2012 PLoS One. 7(1):e29266. Epub 2012 Jan 18; Vector Backbone:pST5552; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacterial replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27027532
Proper citation: RRID:Addgene_78314 Copy
Species: Synthetic
Genetic Insert: FAT FAK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24222685
Proper citation: RRID:Addgene_78303 Copy
Species: Synthetic
Genetic Insert: KRas FAK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21792185
Comments: A polybasic-geranylgeranyl motif (cysteine) adapted from K-Ras, was fused at the carboxy terminus of the Cyto-FAK biosensor (Addgene plasmid 78300) for the generation of KRas–FAK biosensor that can localize at the general membrane area outside DRM regions
Proper citation: RRID:Addgene_78301 Copy
Species: Synthetic
Genetic Insert: flag-GFP 1-10 tetra Cys
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pRK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27295555
Proper citation: RRID:Addgene_78589 Copy
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