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Species: Homo sapiens
Genetic Insert: Synapsin I promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924
Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters
H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko
Proper citation: RRID:Addgene_32582 Copy
Genetic Insert: G-GECO1.1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: The vector is modified from pBAD/ His B (Invitrogen).
Proper citation: RRID:Addgene_32467 Copy
Genetic Insert: G-GECO1.2
Vector Backbone Description: Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: The vector is modified from pBAD/ His B (Invitrogen).
Proper citation: RRID:Addgene_32468 Copy
Species: synthetic construct
Genetic Insert: R-GECO1.0
Vector Backbone Description: Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: The vector is modified from pBAD/ His B (Invitrogen).
Proper citation: RRID:Addgene_32465 Copy
Species: Homo sapiens
Genetic Insert: NRBP
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11956649
Proper citation: RRID:Addgene_32504 Copy
Species: Homo sapiens
Genetic Insert: CIB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11756406
Proper citation: RRID:Addgene_32505 Copy
Genetic Insert: BCR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17116687
Proper citation: RRID:Addgene_32507 Copy
Genetic Insert: Tcf/Lef:H2B-GFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21176145
Comments: To g ene r a t e t h e p T CF /L e f :H 2 B -GFP co n s t ru c t , s i x
copies of the TCF/Lef response elements together with
the hsp68 minimal promoter from the TCF/Lef-LacZ
reporter construct [11] were inserted into the AseI/Nhe1
sites of pCMV::H2B-GFP.
Proper citation: RRID:Addgene_32610 Copy
Species: Homo sapiens
Genetic Insert: RAP1
Vector Backbone Description: Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:15469846
Comments: Q63 changed to E to give phenotype
Proper citation: RRID:Addgene_32698 Copy
Species: Homo sapiens
Genetic Insert: GRK4
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8626439
Comments: Contains the longest splice variant of GRKa. The insert does not contain the 3'UTR.
Proper citation: RRID:Addgene_32690 Copy
Species: Gallus gallus
Genetic Insert: 1XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32614 Copy
Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32612 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32617 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was assembled and may contain minor discrepancies that should not effect the function of the plasmid.
Proper citation: RRID:Addgene_32683 Copy
Genetic Insert: luciferase
Vector Backbone Description: Backbone Marker:Jaensich laboratory; Backbone Size:6120; Vector Backbone:pBS31'-RBGpA; Vector Types:Mouse Targeting, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21458673
Proper citation: RRID:Addgene_32720 Copy
Genetic Insert: mRFP1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15996270
Comments: The mRFP1 coding sequence was amplified by PCR using
primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and
3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx
Polymerase (Invitrogen). The resulting product was
cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene
6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and
verify that it produced robust red fluorescence
More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12)
Proper citation: RRID:Addgene_32600 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21418658
Proper citation: RRID:Addgene_32686 Copy
Genetic Insert: GPI-GFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604528
Proper citation: RRID:Addgene_32601 Copy
Genetic Insert: myr-Venus
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604528
Comments: To generate myristoylated fluorescent fusion proteins, an N-terminal myristoylation tag
derived from Src was generated by adding the sequences MGSSKSKPK to the N-terminus of
any given fluorescent protein. This was achieved by using the following oligonucleotide: 5′
GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA
AAG GTG AGC AAG GGC GAG GAG CTG). The GFP and Venus coding sequences were
amplified from pEGFP-N1 (BD Biosciences, San Jose, CA) and pCS2-Venus (Nagai et al.,
2002) to generate myr-GFP and myr-Venus, respectively, by high-fidelity polymerase chain
reaction (PCR) using Pfx Polymerase (Invitrogen, La Jolla, CA) with the 5′ myristoylation
primer combined with a 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG
TCC) primer, respectively. The resulting product was cloned into the EcoRI site of pCAGGS
to generate pCX::myr-EGFP and pCX::myr-Venus
Proper citation: RRID:Addgene_32602 Copy
Species: Homo sapiens
Genetic Insert: CCND1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Luciferase, reporter gene; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10201372
Proper citation: RRID:Addgene_32726 Copy
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