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Species: Homo sapiens
Genetic Insert: Arf4
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23783033
Proper citation: RRID:Addgene_79407 Copy
Species: Homo sapiens
Genetic Insert: Arf4
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23783033
Proper citation: RRID:Addgene_79406 Copy
Species: Homo sapiens
Genetic Insert: CCL5 Promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4765; Vector Backbone:pDONR221-P1P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human CCL5 Promoter. First Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79492 Copy
Species: Homo sapiens
Genetic Insert: Arf5
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22522702
Proper citation: RRID:Addgene_79534 Copy
Species: Homo sapiens
Genetic Insert: TCF7
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human TCF7 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79498 Copy
Species: Homo sapiens
Genetic Insert: WNT1
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human WNT1 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79499 Copy
Species: Homo sapiens
Genetic Insert: INPP5E
Vector Backbone Description: Backbone Marker:C. Tucker, University of Colorado Denver; Vector Backbone:mCherry-CRY2PHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22847441
Proper citation: RRID:Addgene_79561 Copy
Species: Homo sapiens
Genetic Insert: OCRL
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22847441
Proper citation: RRID:Addgene_79567 Copy
Species: Homo sapiens
Genetic Insert: INPP5E
Vector Backbone Description: Backbone Marker:C. Tucker, University of Colorado Denver; Vector Backbone:mCherry-CRY2PHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22847441
Comments: This construct, lacking the N-terminal fluorescent protein, was generated by Nhe1/Not1 digestion to remove mCherry followed by Mungbean nuclease treatment and blunt end ligation.
Proper citation: RRID:Addgene_79564 Copy
Species: Homo sapiens
Genetic Insert: INPP5E
Vector Backbone Description: Backbone Marker:C. Tucker, University of Colorado Denver; Vector Backbone:mCherry-CRY2PHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22847441
Proper citation: RRID:Addgene_79563 Copy
Species: Homo sapiens
Genetic Insert: OCRL
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_79566 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human YAP1 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79503 Copy
Species: Homo sapiens
Genetic Insert: SMAD3
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human SMAD3 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79507 Copy
Species: Homo sapiens
Genetic Insert: Lyn11
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22847441
Comments: CIBN was fused N-terminally to GFP in pEGFP-N1 using Nhe1/Age1. Complementary oligonucleotides encoding Lyn11 with flanking Nhe1-sites subsequently were annealed and ligated N-terminally to CIBN. Lipidation for plasma membrane attachment occurs at amino acids 2 and 3. The G8R mutations stabilise the protein at the plasma membrane.
Proper citation: RRID:Addgene_79572 Copy
Species: Homo sapiens
Genetic Insert: PGK Promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4765; Vector Backbone:pDONR221-P1P5r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for human PGK Promoter. First Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79489 Copy
Species: Homo sapiens
Genetic Insert: CRACR2A-isoform-a
Vector Backbone Description: Backbone Size:7480; Vector Backbone:MO70; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27016526
Comments: This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference
Proper citation: RRID:Addgene_79592 Copy
Species: Homo sapiens
Genetic Insert: LXR Alpha
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:4774; Vector Backbone:pDONR221-P5P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694838
Comments: Multisite Gateway Entry Clone for NR1H3 CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
Proper citation: RRID:Addgene_79514 Copy
Species: Homo sapiens
Genetic Insert: EMG1
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please see http://www.thesgc.org/structures/5FAI for more information.
Proper citation: RRID:Addgene_79519 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 78173); Vector Backbone:pET His10 TEV LIC cloning vector (2B-T-10); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30004690
Comments: This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
Proper citation: RRID:Addgene_79708 Copy
Species: Homo sapiens
Genetic Insert: CSK21
Vector Backbone Description: Backbone Marker:Scott Gradia (Addgene plasmid # 78173); Vector Backbone:pET His10 TEV LIC cloning vector (2B-T-10); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30004690
Comments: This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/
Proper citation: RRID:Addgene_79707 Copy
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