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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4850; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000086; Origin: SC101
Proper citation: RRID:Addgene_35320 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4031; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000095; Origin: SC101
Proper citation: RRID:Addgene_35329 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5520; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: ADE2 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Ade2 protein.
Proper citation: RRID:Addgene_35434 Copy
Species: Synthetic
Genetic Insert: SnAvi-tag
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_35399 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3642; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000082; Origin: p15A
Proper citation: RRID:Addgene_35316 Copy
Species: Mus musculus
Genetic Insert: Mycn
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35393 Copy
Species: Synthetic
Genetic Insert: TALE-Oct4
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_35391 Copy
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:13844; Vector Backbone:pDW1714; Vector Types:Yeast Expression, reporter; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21493687
Comments: Cloning nuclease targets into the BglII/SpeI sites of pCP5 removes the CcdB gene, thus allows negative selection against undigested backbone in common E. coli strains such as DH5-alpha.
Proper citation: RRID:Addgene_35397 Copy
Species: Mus musculus
Genetic Insert: Myc
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35396 Copy
Species: Mus musculus
Genetic Insert: Myc
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35395 Copy
Species: Mus musculus
Genetic Insert: Mycn
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22340591
Proper citation: RRID:Addgene_35394 Copy
Species: Synthetic
Genetic Insert: TALE-Sox2
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Proper citation: RRID:Addgene_35388 Copy
Species: Synthetic
Genetic Insert: TALE6
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Comments: Note that though this plasmid contains the sh_ble gene, the Zhang lab has not tested whether this plasmid confers Zeocin selection.
Proper citation: RRID:Addgene_35421 Copy
Species: Homo sapiens
Genetic Insert: Protein Kinase C iota
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4
Proper citation: RRID:Addgene_35387 Copy
Species: Synthetic
Genetic Insert: TALE5
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Comments: Note that though this plasmid contains the sh_ble gene, the Zhang lab has not tested whether this plasmid confers Zeocin selection.
Proper citation: RRID:Addgene_35420 Copy
Species: Synthetic
Genetic Insert: TALE12
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21248753
Comments: Note that though this plasmid contains the sh_ble gene, the Zhang lab has not tested whether this plasmid confers Zeocin selection.
Proper citation: RRID:Addgene_35427 Copy
Species: Homo sapiens
Genetic Insert: hPLK2 K111M
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2K111M and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35386 Copy
Species: Homo sapiens
Genetic Insert: hPLK2
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2WT and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35385 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2832; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000260; Origin: BBR1
Proper citation: RRID:Addgene_35371 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2774; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000261; Origin: BBR1
Proper citation: RRID:Addgene_35370 Copy
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