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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMV-luc-miR21(P) Resource Report Resource Website 1+ mentions |
RRID:Addgene_20382 | mir21(P) targets | Ampicillin | PMID:12554881 | Backbone Size:5200; Vector Backbone:pCMV-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:57 | 1 | |||
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pTC-0X1L Resource Report Resource Website 1+ mentions |
RRID:Addgene_20315 | Pmyc1tetO-lacZ | E. coli | Kanamycin | PMID:19174563 | Backbone Size:4103; Vector Backbone:pTC-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:01:52 | 1 | ||
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pSirt6-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_20275 | Sirt6 | Mus musculus | Kanamycin | PMID:15795229 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:01:52 | 1 | ||
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pKEN GFP mut2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20409 | GFP | Aequorea victoria | Ampicillin | PMID:8707053 | An XbaI site was introduced at the beginning of GFP, see attached map for more details. The protein is expressed from IPTG inducible promoter in pKEN vector. GFP is cloned as XbaI-PstI fragment into the polylinker; there is also NdeI site at the AUG that can be used. Mut 2 is probably best for expression in bacteria. Overexpression of GFP renders the cells osmosensitive. In the absence of IPTG, DH12 carrying the enclosed plasmids will lyse in LB. We recommend growing this plasmid in low salt LB (<5g/L NaCl); alternatively use E.coli strain that grossly overproduces lacI and totally shuts expression down. | Backbone Marker:Ezaz-Nikpay et al, 1994 Protein Science; Backbone Size:3531; Vector Backbone:pKEN; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S65A V68L S72A | 2026-08-29 01:01:55 | 2 |
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pESC-URA-GFP-Ubc9wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_20368 | SUMO-conjugating enzyme Ubc9 | Saccharomyces cerevisiae | Ampicillin | PMID:18756251 | Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:53 | 2 | ||
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mycUBB+1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20254 | Ubiquitin B +1 transcript | Homo sapiens | Ampicillin | PMID:18796559 | Backbone Size:3300; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:52 | 1 | ||
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GST TopBP1 (aa 32-1522) His Resource Report Resource Website 1+ mentions |
RRID:Addgene_20375 | TopBP1 | Homo sapiens | Ampicillin | PMID:19139065 | TopBP1 cDNA KIAA00259 was cloned by Weei-Chin Lin in EcoRI site of pGEX6P1. I added a His6 tag at the 3' end using the oligos TopBPF3 and TopBPR4 for PCR, then digested the PCR product with XhoI and replaced the XhoI fragment from pGEX6P1TopBP1 with the PCR product to generate pGEX6P1TopBP1His. NOTE: This plasmid was originally called full-length, and it is considered to have full length functionality in its original publication from the Sancar lab. | Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Starts at aa32 | 2026-08-29 01:01:56 | 4 |
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pCDNA3/GFP-PR Resource Report Resource Website 1+ mentions |
RRID:Addgene_20253 | HIV-1 protease | human immunodeficiency virus type 1 | Ampicillin | PMID:11502538 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:56 | 3 | ||
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Luc-SIRT1 3'UTR Resource Report Resource Website 1+ mentions |
RRID:Addgene_20379 | SIRT1 | Homo sapiens | Ampicillin | PMID:18755897 | There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function. | Backbone Size:6470; Vector Backbone:pMIR-REPORT; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 3'UTR of SIRT1 | 2026-08-29 01:01:57 | 4 |
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pWZL Neo Myr Flag HK2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20501 | HK2 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:56 | 4 | |
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pWZL Neo Myr Flag MAP2K7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20516 | MAP2K7 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:56 | 1 | |
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pWZL Neo Myr Flag CDK5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20449 | CDK5 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:55 | 1 | |
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pWZL Neo Myr Flag CAMK4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20441 | CAMK4 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:55 | 1 | |
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pWZL Neo Myr Flag PACSIN1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20545 | PACSIN1 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:56 | 1 | |
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pWZL Neo Myr Flag AXL Resource Report Resource Website 1+ mentions |
RRID:Addgene_20428 | AXL | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:53 | 5 | |
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pWZL Neo Myr Flag AKT3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20423 | AKT3 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:53 | 1 | |
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pWZL Neo Myr Flag AKT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_20422 | AKT1 | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:57 | 4 | |
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pWZL Neo Myr Flag CAMK1G Resource Report Resource Website 1+ mentions |
RRID:Addgene_20438 | CAMK1G | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:57 | 1 | |
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pWZL Neo Myr Flag BLK Resource Report Resource Website 1+ mentions |
RRID:Addgene_20430 | BLK | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:55 | 1 | |
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pWZL Neo Myr Flag BTK Resource Report Resource Website 1+ mentions |
RRID:Addgene_20432 | BTK | Homo sapiens | Ampicillin | PMID:17574021 | ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 | Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:01:53 | 2 |
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