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Species: Saccharomyces cerevisiae
Genetic Insert: mutant HSP104
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC25S; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158570
Proper citation: RRID:Addgene_1300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/mCherry-AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL11B
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS1530; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11071906
Comments: CEN vector with ORF of GFP followed by the 3'UTR of NUF2.
Proper citation: RRID:Addgene_1364 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: RPL25 promoter and RPL25 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1369 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-rhaP-BAD-malE-His tag-smt3-eGFP
Vector Backbone Description: Vector Backbone:pTST101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19127343
Proper citation: RRID:Addgene_135085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CMVpr hph ADH1t
Vector Backbone Description: Backbone Size:4287; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32392127
Proper citation: RRID:Addgene_135414 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpunHsp70pr hph-GFP ADH1t
Vector Backbone Description: Backbone Size:9369; Vector Backbone:pGI3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135482 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpunH2Bpr hph-GFP ADH1t
Vector Backbone Description: Backbone Size:9576; Vector Backbone:pGI3; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135483 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hph SpunH2A/Bpr hph-mCitrine
Vector Backbone Description: Backbone Size:10906; Vector Backbone:pGI3EM18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: mCitrine was obtained by PCR of mCitrine-PCNA-19-SV40NLS-4 (Addgene plasmid # 56564), a plasmid created by the Davidson lab
The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135489 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hph SpunH2A/Bpr hph-mClover3
Vector Backbone Description: Backbone Size:10906; Vector Backbone:pGI3EM18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32392127
Comments: mClover3 was obtained by PCR of pKK-mClover3-TEV (Addgene plasmid \# 105778), a plasmid created by the Dziembowski lab
The pGI3 vector was a gift from Giuseppe Ianiri
Proper citation: RRID:Addgene_135490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KAP123
Vector Backbone Description: Backbone Size:7300; Vector Backbone:pPS934; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9238021
Comments: KAP123 replaces NUF2 of pPS934, CEN plasmid.
Proper citation: RRID:Addgene_1355 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR6
Vector Backbone Description: Vector Backbone:pSE358; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31558568
Proper citation: RRID:Addgene_135590 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR6
Vector Backbone Description: Vector Backbone:pSE358; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31558568
Proper citation: RRID:Addgene_135586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: snR6
Vector Backbone Description: Vector Backbone:pSE358; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31558568
Proper citation: RRID:Addgene_135587 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GSP1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS293; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7816822
Comments: pPS293 has the GAL1 promoter (EcoRI/XbaI fragment) in YEp352 (2 micron ori).
Proper citation: RRID:Addgene_1357 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GSP1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS293; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7816822
Comments: pPS293 has the GAL1 promoter (EcoRI/XbaI fragment) in YEp352 (2 micron ori).
Proper citation: RRID:Addgene_1358 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGYP7-YFP
Vector Backbone Description: Vector Backbone:pYTK143; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32898473
Proper citation: RRID:Addgene_133120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mScarlet-CLASP
Vector Backbone Description: Vector Backbone:pYTK146; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32898473
Proper citation: RRID:Addgene_133086 Copy
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