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Species: Synthetic
Genetic Insert: 35S:MS2-KYP:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-KYP coding sequence = 1542-2834bp
RBCS terminator = 2839-3484bp
BsaI site #1 = 3519-3524bp
BsaI site #2 = 3533-3538bp
Proper citation: RRID:Addgene_122865 Copy
Species: Synthetic
Genetic Insert: p35S:MS2-GFP:tRBCS
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31557222
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules.
Plasmid Features (listed as bp in full plasmid sequence):
35S promoter = 656-1502bp
MS2-GFP coding sequence = 1542-2846bp
RBCS terminator = 2851-3496bp
BsaI site #1 = 3531-3536bp
BsaI site #2 = 3545-3550bp
Proper citation: RRID:Addgene_122859 Copy
Species: Homo sapiens
Genetic Insert: Gamma-aminobutyric acid receptor-associated protein-like 1
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123085 Copy
Species: Homo sapiens
Genetic Insert: Gamma-aminobutyric acid receptor-associated protein
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123081 Copy
Species: Homo sapiens
Genetic Insert: Gamma-aminobutyric acid receptor-associated protein
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123080 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123072 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3A
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123071 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123074 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3 beta 2
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123075 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3 beta 2
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123077 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3C
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123079 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3A
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123070 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3A
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123069 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3C
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123208 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3A
Vector Backbone Description: Backbone Size:2796; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123204 Copy
Species: E.coli
Genetic Insert: tetR gene by pFRA61 (Boldrin et al 2010)
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pSM128 derived plasmid (the region carrying the attB site and the integrase gene was previously removed using NheI and HindIII); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30962489
Comments: The plasmid also carries attP sequence belonging to pMC1s plasmid and PfurA 102 tetO- pip belonging to pFRA61 (Boldrin et al 2010).
Proper citation: RRID:Addgene_131106 Copy
Genetic Insert: oxySp-mCherry-TEVrs-LAA-TermT1
Vector Backbone Description: Backbone Size:2055; Vector Backbone:pZA4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31492904
Proper citation: RRID:Addgene_132586 Copy
Species: Arabidopsis thaliana
Genetic Insert: egg cell promoter, Cas9-mApple, pds3 sgRNA
Vector Backbone Description: Backbone Marker:https://gateway.psb.ugent.be; Backbone Size:8820; Vector Backbone:pH2GW7; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32096870
Comments: Egg cell promoters amplified directly from A. thaliana leaf tissue based on the promoters in Addgene plasmid #71288, described in doi: 10.1186/s13059-015-0715-0 Please visit https://www.biorxiv.org/content/10.1101/852020v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_132568 Copy
Genetic Insert: phytoene-beta carotene synthase
Vector Backbone Description: Vector Backbone:LCB7; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: The minor differences between the sequence and .gb file are of no functional concern.
Proper citation: RRID:Addgene_140232 Copy
Genetic Insert: dCas9 & Csy4
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section.
Proper citation: RRID:Addgene_140664 Copy
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