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Species: Drosophila melanogaster
Genetic Insert: CG17293
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71079 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71228 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note-This plasmid was originally reported to have a C-terminal frameshift mutation in RebB (Polka et al ACS Synth Biol. 2016).. Addgene's quality control sequences indicate that the frameshift mutation is not present and the plasmid contains the entire RebB gene.
Proper citation: RRID:Addgene_71229 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71226 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: formyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22374910
Comments: An unexplained deletion event removed the F1 ori, M13 ori region, the T7 terminator, and C-terminal His-tag region. Instead we see a sequence of 29 nt that does not correspond to any obvious source. The net plasmid is 629 nt shorter than expected. The major practical consequence of this is there is no terminator -- of any kind -- oriented parallel to the expressed gene. The expressed protein ORF appears to be completely correct.
Proper citation: RRID:Addgene_71121 Copy
Species: Acetobacter aceti 1023
Genetic Insert: formyl-CoA:oxalate CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22374910
Proper citation: RRID:Addgene_71120 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71232 Copy
Species: Other
Genetic Insert: Reb locus
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26814170
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_71230 Copy
Species: Mus musculus
Genetic Insert: Grp1-PH
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9804818
Proper citation: RRID:Addgene_71378 Copy
Species: Synthetic
Genetic Insert: 2PH-PLC delta (Human)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR 221; Vector Types:Gateway multisite middle entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26585296
Proper citation: RRID:Addgene_71283 Copy
Species: Homo sapiens
Genetic Insert: STIMATE (1-240)
Vector Backbone Description: Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26322679
Proper citation: RRID:Addgene_71280 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR, Plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26193878
Comments: Please see the cloning protocol attachments for detailed descriptions on how to insert sgRNA sequences into this plasmid.
Proper citation: RRID:Addgene_71287 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting TRY and CPC genes
Vector Backbone Description: Vector Backbone:pCambia; Vector Types:Plant Expression, CRISPR, Plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26193878
Comments: TRY & CPC sgRNA target sequence is: AATATCTCTCTATCTCCTC
ETC2 sgRNA target sequence is: GAAGTGAGTAGCATCGAAT
See Figure 1B of the associated publication for more details.
Proper citation: RRID:Addgene_71288 Copy
Species: Synthetic
Genetic Insert: NADPH Alanine Dehydrogenase
Vector Backbone Description: Backbone Marker:Lucigen (Middleton, WI); Backbone Size:1800; Vector Backbone:pSMART-HCKan (AF532107.1); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34600151
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
Proper citation: RRID:Addgene_71326 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pIRES-hrGFP II; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20627111
Proper citation: RRID:Addgene_71317 Copy
Species: changed Cysteine 176 to Alanine
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71360 Copy
Species: Homo sapiens
Genetic Insert: cortactin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469447
Comments: silent mutation present at 72nd nucleotide
Proper citation: RRID:Addgene_71365 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469447
Proper citation: RRID:Addgene_71366 Copy
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Comments: Please Note: the HP0518 insert has a C-terminal His tag.
Proper citation: RRID:Addgene_71353 Copy
Genetic Insert: HP0518
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET28(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26306031
Proper citation: RRID:Addgene_71351 Copy
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