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Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15596010
Proper citation: RRID:Addgene_32710 Copy
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pISce1; Vector Types:Zebrafish Transgenesis; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32674 Copy
Species: Homo sapiens
Genetic Insert: IRF3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20382888
Proper citation: RRID:Addgene_32713 Copy
Species: Homo sapiens
Genetic Insert: TLR3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20382888
Comments: Please note that the E127G point mutation does not affect the function of this plasmid.
Proper citation: RRID:Addgene_32712 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15596010
Proper citation: RRID:Addgene_32709 Copy
Species: Mus musculus
Genetic Insert: Kir2.1-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32669 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138822
Proper citation: RRID:Addgene_32703 Copy
Species: Homo sapiens
Genetic Insert: GRP78/BiP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11375416
Comments: The D238G point mutation found in the Addgene sequence is a known variation in human GRP78 that does not alter its chaperone or ATPase activities.
Proper citation: RRID:Addgene_32701 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15596010
Proper citation: RRID:Addgene_32707 Copy
Genetic Insert: emGFP-miRNA
Vector Backbone Description: Backbone Marker:Richard O. Hynes group; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138822
Proper citation: RRID:Addgene_32704 Copy
Species: Mus musculus
Genetic Insert: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1/Hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11457730
Proper citation: RRID:Addgene_32705 Copy
Genetic Insert: FLPo
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32650 Copy
Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.
Proper citation: RRID:Addgene_32641 Copy
Species: Mus musculus
Genetic Insert: tetO-Kir2.1-IRES-TLZ
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide a single nucleotide deletion at bp#1135 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.
Proper citation: RRID:Addgene_32642 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32648 Copy
Genetic Insert: ChR2
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32646 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32649 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32594 Copy
Genetic Insert: H2B-EGFP
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15619330
Comments: Please note that the plasmid contains a duplication of the H2B-EGFP region as compared to the original depositor sequence. The depositor has confirmed that the plasmid functions as described in the associated publication.
The coding sequence for the human histone H2B gene
(X57127) was amplified from genomic DNA by PCR
using Pfx Polymerase (Invitrogen). The resulting product
was cloned into pCR4 TOPO (Invitrogen) to generate
pH2B. The H2B fragment was then cloned into plasmids
pEGFP-N1, pDsRed2-N1 pDsRedExpress-N1 (BD Biosciences, Inc) in order to generate plasmids pH2B-EGFP,
pH2B-DsRed2 and pH2B-DsRedExpress (oligonucleotide
sequences are available upon request). The resulting
fusions were then re-amplified by PCR and cloned into
the XhoI site of pCAGGS [19] to generate pCX-H2B-EGFP,
pCX-H2B-DsRed2 and pCX-H2B-DsRedExpress.
Proper citation: RRID:Addgene_32599 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12079497
Proper citation: RRID:Addgene_32597 Copy
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