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Species: Synthetic
Genetic Insert: Glucoamylase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84969 Copy
Species: Synthetic
Genetic Insert: Alcohol oxidase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84960 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85042 Copy
Species: Synthetic
Genetic Insert: mScarlet-H
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85043 Copy
Species: Synthetic
Genetic Insert: Pichia autonomously replicating sequence
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84990 Copy
Species: Synthetic
Genetic Insert: Alcohol oxidase 1 terminator
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84988 Copy
Species: Synthetic
Genetic Insert: BxbI recognition site
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84989 Copy
Species: Synthetic
Genetic Insert: PA50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84906 Copy
Species: Synthetic
Genetic Insert: PR50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84905 Copy
Species: Synthetic
Genetic Insert: GA50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84907 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Gadella Lab; Backbone Size:4693; Vector Backbone:pmScarlet_N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85054 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5329; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85093 Copy
Species: Synthetic
Genetic Insert: DNA polymerase sliding clamp 3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PCNA3 gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85094 Copy
Species: Synthetic
Genetic Insert: Pro-Pro-Pro-Pro-Pro
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Proper citation: RRID:Addgene_85098 Copy
Species: Synthetic
Genetic Insert: NES
Vector Backbone Description: Backbone Marker:Gadella Lab; Backbone Size:4710; Vector Backbone:pmScarlet_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85060 Copy
Species: Synthetic
Genetic Insert: NES
Vector Backbone Description: Backbone Marker:Gadella Lab; Backbone Size:4710; Vector Backbone:pmScarlet-i_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85062 Copy
Species: Synthetic
Genetic Insert: NES
Vector Backbone Description: Backbone Marker:Gadella Lab; Backbone Size:4710; Vector Backbone:pmScarlet-H_C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85061 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Comments: A synthetic gene of PdX was inserted between EcoRI and BamHI sites of pHSG398 using In-Fusion cloning kit.
Proper citation: RRID:Addgene_85100 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Gadella Lab; Backbone Size:4693; Vector Backbone:pmScarlet-i_N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_85056 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PdX gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85084 Copy
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