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Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17013387
Proper citation: RRID:Addgene_24454 Copy
Species: Homo sapiens
Genetic Insert: Kinesin
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17013387
Proper citation: RRID:Addgene_24451 Copy
Species: Homo sapiens
Genetic Insert: mir-20
Vector Backbone Description: Backbone Size:7651; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20008935
Proper citation: RRID:Addgene_24709 Copy
Species: Homo sapiens
Genetic Insert: Prickle 2
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pGateway 5'CMV Trible Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24645 Copy
Species: Homo sapiens
Genetic Insert: Prickle 1
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pGateway 5'CMV Trible Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24644 Copy
Species: Homo sapiens
Genetic Insert: SGK3
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16790420
Proper citation: RRID:Addgene_24650 Copy
Species: Homo sapiens
Genetic Insert: NFATc4
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS.KS+; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10537109
Proper citation: RRID:Addgene_24586 Copy
Species: Homo sapiens
Genetic Insert: Yap2
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24637 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24605 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24604 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24607 Copy
Species: Homo sapiens
Genetic Insert: Smurf2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20026602
Proper citation: RRID:Addgene_24606 Copy
Species: Homo sapiens
Genetic Insert: WNK2
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1/V5–His–TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17578925
Proper citation: RRID:Addgene_24569 Copy
Species: Homo sapiens
Genetic Insert: human XIST
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2686; Vector Backbone:puc18; Vector Types:plasmid cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8636206
Proper citation: RRID:Addgene_24690 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24806 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24809 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24803 Copy
Species: Homo sapiens
Genetic Insert: DVL2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19379695
Proper citation: RRID:Addgene_24802 Copy
Species: Homo sapiens
Genetic Insert: TGFbeta receptor II
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: HA tag: PYDVPDYA
Proper citation: RRID:Addgene_24801 Copy
Species: Homo sapiens
Genetic Insert: Pirh2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX-2TKm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043414
Comments: E. coli strain for expression: BL21DE3
Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.
Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.
For pGEX-2TKm vector, the kinase site RRASV is removed and NdeI site is inserted at 5' of BamHI.
Proper citation: RRID:Addgene_24882 Copy
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