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Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24814 Copy
Species: Homo sapiens
Genetic Insert: p35
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11813240
Proper citation: RRID:Addgene_24895 Copy
Species: Homo sapiens
Genetic Insert: shRNA-GFP
Vector Backbone Description: Backbone Marker:Halo-Bio RNAi Therapeutics; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16611886
Comments: shRNA oligo sequence is - 5′-GCAAGCTGACCCTGAAGTTC
Proper citation: RRID:Addgene_24747 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24868 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24749 Copy
Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553310
Proper citation: RRID:Addgene_24744 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24864 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24860 Copy
Species: Homo sapiens
Genetic Insert: Rabin8
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24910 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24870 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24738 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24859 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24735 Copy
Species: Homo sapiens
Genetic Insert: BRaf-ER
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS no LacZ; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11896581
Proper citation: RRID:Addgene_24949 Copy
Species: Homo sapiens
Genetic Insert: p16
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe puro3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18394558
Proper citation: RRID:Addgene_24934 Copy
Species: Homo sapiens
Genetic Insert: p18
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe puro3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18394558
Proper citation: RRID:Addgene_24936 Copy
Species: Homo sapiens
Genetic Insert: HES1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Proper citation: RRID:Addgene_24982 Copy
Species: Homo sapiens
Genetic Insert: mir-9-3
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen, Addgene plasmid 11375; Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: The mir-9-3 gene was PCR-amplified from normal human genomic DNA and cloned into the MDH-PGK-GFP 2.0 retroviral vector. It includes the miR-9 stem-loop and ~100bp flanking sequences on both sides.
Proper citation: RRID:Addgene_25036 Copy
Species: Homo sapiens
Genetic Insert: CDH1 mutant 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20173740
Comments: A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3′ UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).
Proper citation: RRID:Addgene_25039 Copy
Species: Homo sapiens
Genetic Insert: STAT3
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19074878
Comments: A V667L mutation is present but not known to affect function.
Expression of EGFP is driven by the human ubiquitin promoter. See Addgene plasmid 14883: FUGW for more information on the empty vector.
Proper citation: RRID:Addgene_24983 Copy
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