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Species: Synthetic
Genetic Insert: Putidaredoxin reductase
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5333; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The PdR gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.
Proper citation: RRID:Addgene_85083 Copy
Species: Synthetic
Genetic Insert: The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: A synthetic gene of PdR was inserted at the NdeI site of p1G108CP20X (Plasmid #85096) using In-Fusion cloning kit.
Proper citation: RRID:Addgene_85086 Copy
Species: Synthetic
Genetic Insert: PCNA3 fused to P450cam
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5332; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27781345
Comments: The plamid was obtained from pGS-3 (Plasmid #85094) and pBsaP450camGG (Plasmid #85101) by Golden Gate assembly.
Proper citation: RRID:Addgene_85087 Copy
Species: Synthetic
Genetic Insert: GeNL(Ca2+)_250
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Proper citation: RRID:Addgene_85206 Copy
Species: Synthetic
Genetic Insert: GeNL(Ca2+)_60
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Proper citation: RRID:Addgene_85207 Copy
Species: Synthetic
Genetic Insert: Green enhanced Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Proper citation: RRID:Addgene_85200 Copy
Species: Synthetic
Genetic Insert: Yellow enhanced Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Proper citation: RRID:Addgene_85201 Copy
Species: Synthetic
Genetic Insert: Optimized G Protein
Vector Backbone Description: Vector Backbone:pAAV-syn-FLEX-splitTVA-eGFP-B19G IW; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_85225 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Size:6417; Vector Backbone:LeGO vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28109958
Comments: HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de
The BsrGI site of EBFP2 can be used to fuse an antibiotic resistance to the fluorescent protein following the building block principle of the LeGO vector system. More information is available on the LeGO website and in the following publications.
More references:
Weber et al., Molecular Therapy, 2008:
http://www.nature.com/mt/journal/v16/n4/full/mt20086a.html
Weber et al., Gene Therapy, 2010:
http://www.nature.com/gt/journal/v17/n4/full/gt2009149a.html
Proper citation: RRID:Addgene_85213 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pRS402; Vector Types:Yeast Expression, integrating, shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22722195
Proper citation: RRID:Addgene_85190 Copy
Species: Synthetic
Genetic Insert: Cyan enhanced Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27966527
Proper citation: RRID:Addgene_85199 Copy
Species: Synthetic
Genetic Insert: rhodopsin-guanylyl cyclase
Vector Backbone Description: Backbone Size:3045; Vector Backbone:pGEM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26268609
Proper citation: RRID:Addgene_85469 Copy
Species: Synthetic
Genetic Insert: iChloC
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26443033
Proper citation: RRID:Addgene_85467 Copy
Species: Synthetic
Genetic Insert: Bacterial Photoactivated Adenylyl Cyclase
Vector Backbone Description: Backbone Size:3152; Vector Backbone:pGEM; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21030594
Proper citation: RRID:Addgene_85468 Copy
Species: Synthetic
Genetic Insert: Synthetic construct PsChR1 gene
Vector Backbone Description: Backbone Size:4703; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26740624
Proper citation: RRID:Addgene_85465 Copy
Species: Synthetic
Genetic Insert: Synthetic construct ACR2 gene
Vector Backbone Description: Backbone Size:4703; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26740624
Proper citation: RRID:Addgene_85463 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/Myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85482 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-IRES-GFP
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8812; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27708057
Proper citation: RRID:Addgene_85556 Copy
Species: Synthetic
Genetic Insert: Sp dCas9
Vector Backbone Description: Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27984730
Comments: Multicolor CRISPR labeling of chromosomal loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. 10.1073/pnas.1420024112 PubMed 25713381
Proper citation: RRID:Addgene_85717 Copy
Species: Synthetic
Genetic Insert: 1xNLS Mislocaliser
Vector Backbone Description: Vector Backbone:pGEM; Vector Types:P. falciparum Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28288121
Proper citation: RRID:Addgene_85795 Copy
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