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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4782; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000104; Origin: SC101
Proper citation: RRID:Addgene_35338 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4186; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000096; Origin: SC101
Proper citation: RRID:Addgene_35330 Copy
Species: Homo sapiens
Genetic Insert: PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25)
Vector Backbone Description: Backbone Marker:MBL International; Backbone Size:4244; Vector Backbone:pAsh-MCL; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33890764
Proper citation: RRID:Addgene_178871 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11^SEC^3xMyc
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).
Proper citation: RRID:Addgene_178877 Copy
Genetic Insert: YC3.60
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9215; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Comments: Please note: Plasmid contains N165H and K213N mutations in YC3.60 and has a Ser3 deletion in Cerulean. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_178904 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7446; Vector Backbone:pFastBac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178816 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6696; Vector Backbone:pFastBac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178818 Copy
Species: Rippkaea orientalis
Genetic Insert: partial tRNA-Leugene from Rippkaea orientalis
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178774 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas5
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178778 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas6
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178779 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cse2
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178777 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3700; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178898 Copy
Species: Rippkaea orientalis
Genetic Insert: Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178771 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2510; Vector Backbone:BASIC_SEVA_39.10; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178926 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-F1 cas2-3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178769 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7404; Vector Backbone:pFastBac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178806 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7401; Vector Backbone:pFastBac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178807 Copy
Species: Marinomonas sp.
Genetic Insert: gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178764 Copy
Species: Homo sapiens
Genetic Insert: human rhinovirus 3C protease
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178885 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2654; Vector Backbone:BASIC_SEVA_29.10; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178921 Copy
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