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Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155221 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155222 Copy
Vector Backbone Description: Backbone Size:9512; Vector Backbone:Addgene #155182; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Comments: The T2A-Puro sequence was re-amplified and cloned into the backbone to remove BbsI restriction site in the T2A sequence. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns and Ms are the gRNA sequence and its reverse-complement counterpart:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_155184 Copy
Species: Homo sapiens
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155220 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15553 Copy
Species: Mus musculus
Genetic Insert: Wdr5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15552 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15551 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17500065
Proper citation: RRID:Addgene_15550 Copy
Species: Homo sapiens
Genetic Insert: FKBP12(V36)-p30Caspase9
Vector Backbone Description: Backbone Size:5300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728125
Comments: Based on L. Fan et al (99) Human Gene Therapy
Proper citation: RRID:Addgene_15567 Copy
Species: Synthetic
Genetic Insert: H11-H4
Vector Backbone Description: Vector Backbone:pOPINO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32661423
Proper citation: RRID:Addgene_155364 Copy
Species: Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens
Genetic Insert: dCas13-M3nls
Vector Backbone Description: Vector Backbone:pCMV with pBR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32601430
Proper citation: RRID:Addgene_155366 Copy
Species: Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens
Genetic Insert: dCas13-M3M14nes
Vector Backbone Description: Vector Backbone:pCMV with pBR322 origin; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32601430
Proper citation: RRID:Addgene_155367 Copy
Species: Homo sapiens
Genetic Insert: FOXC2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17537911
Proper citation: RRID:Addgene_15535 Copy
Vector Backbone Description: Backbone Size:7562; Vector Backbone:pBigT; Vector Types:Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657333
Comments: Modification of plasmid pBigT described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. For use with pRosa26PA. See BMC Dev Biol article for more information.
Modified to contain IRES-EGFP downstream of cloning site. See Author's Map for more information.
Proper citation: RRID:Addgene_15037 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' and 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6207484
Comments: Vector with Xenopus 5' and 3' untranslated beta-globin sequences to enhance translation of synthetic mRNAs. Cloning site for mRNA is BglII. See author's map for more information.
Proper citation: RRID:Addgene_15030 Copy
Species: Homo sapiens
Genetic Insert: Brca1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10550055
Comments: pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
Proper citation: RRID:Addgene_14999 Copy
Species: Mus musculus
Genetic Insert: Mer
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15673687
Comments: The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.
Proper citation: RRID:Addgene_14998 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12150994
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14966 Copy
Species: Homo sapiens
Genetic Insert: Smad3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12150994
Comments: See author's map for more information.
Proper citation: RRID:Addgene_14967 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (-109/+334)
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pBV-luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158583
Comments: XhoI to PvuII of c-myc promoter was subcloned into pBV-Luc, a low basal activity luciferase reporter plasmid.
Proper citation: RRID:Addgene_14969 Copy
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