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Species: Homo sapiens
Genetic Insert: ZNF219 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Addgene's quality control sequencing finds a G to E amino acid residue substitution in the linker between ZNF219 and the 3xFlag. This residue change is not thought to affect plasmid function. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72367 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72403 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72318 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72316 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72382 Copy
Species: Acetobacter aceti 1023
Genetic Insert: putative CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72425 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72308 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72320 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72321 Copy
Species: Photinus pyralis
Genetic Insert: Photinus pyralis firefly luciferin sulfotransferase
Vector Backbone Description: Backbone Marker:Produced in Jing-Ke Weng lab; Backbone Size:5350; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27227579
Comments: Expressed robustly in E.coli. Catalyzes sulfonation / desulfonation of firefly luciferin / sulfoluciferin with PAPS / PAP.
Proper citation: RRID:Addgene_74121 Copy
Species: Photinus pyralis
Genetic Insert: Photinus pyralis sulfotransferase 3
Vector Backbone Description: Backbone Marker:Produced in Jing-Ke Weng lab; Backbone Size:5350; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27227579
Comments: Expressed robustly in E.coli. High activity for desulfonation of p-nitrophenolsulfate with PAP
Proper citation: RRID:Addgene_74123 Copy
Species: Other
Genetic Insert: Photinus pyralis sulfotransferase 2
Vector Backbone Description: Backbone Marker:Produced in Jing-Ke Weng lab; Backbone Size:5350; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27227579
Comments: Expresses robustly in E.coli. Minimal activity for desulfonation of p-nitrophenolsulfate with PAP
Proper citation: RRID:Addgene_74122 Copy
Species: Homo sapiens
Genetic Insert: PHGDH
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26831078
Proper citation: RRID:Addgene_74113 Copy
Species: Bos taurus
Genetic Insert: Lactadherin-C2
Vector Backbone Description: Backbone Size:4646; Vector Backbone:mRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18187657
Proper citation: RRID:Addgene_74061 Copy
Species: Rattus norvegicus
Genetic Insert: Arrestin3
Vector Backbone Description: Backbone Marker:gift from M. Bouvier (University of Montreal); Vector Backbone:pcDNA3.1-RLucC2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27007854
Proper citation: RRID:Addgene_74132 Copy
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Michael Davidson Lab; Backbone Size:3900; Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879144
Comments: The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.
Proper citation: RRID:Addgene_74253 Copy
Species: Rattus norvegicus
Genetic Insert: Arrestin3
Vector Backbone Description: Backbone Marker:gift from M. Bouvier (University of Montreal); Vector Backbone:pcDNA3.1-RLucC2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27007854
Proper citation: RRID:Addgene_74131 Copy
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Michael Davidson Lab; Backbone Size:3900; Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879144
Comments: The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.
Proper citation: RRID:Addgene_74256 Copy
Genetic Insert: actin
Vector Backbone Description: Backbone Marker:Michael Davidson Lab; Backbone Size:3900; Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879144
Comments: The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.
Proper citation: RRID:Addgene_74255 Copy
Species: Rattus norvegicus
Genetic Insert: Arrestin3
Vector Backbone Description: Backbone Marker:gift from M. Bouvier (University of Montreal); Vector Backbone:pcDNA3.1-RLucC2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27007854
Proper citation: RRID:Addgene_74133 Copy
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