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Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Size:5756; Vector Backbone:pVETL-Cmcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17693639
Proper citation: RRID:Addgene_22121 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 2 alpha
Vector Backbone Description: Backbone Size:5573; Vector Backbone:pBabe-Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14691554
Proper citation: RRID:Addgene_22134 Copy
Species: Homo sapiens
Genetic Insert: Hypoxia inducible factor 2 alpha
Vector Backbone Description: Backbone Size:5188; Vector Backbone:pBabe-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14691554
Proper citation: RRID:Addgene_22135 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Size:5756; Vector Backbone:pVETL-Cmcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17693639
Proper citation: RRID:Addgene_22119 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22117 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22113 Copy
Species: Homo sapiens
Genetic Insert: SA-Puro
Vector Backbone Description: Backbone Size:5890; Vector Backbone:N/A; Vector Types:ZFN donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Comments: Please note that this plasmid contains a non-functional ccdB gene. The use of ccdB survival cells may be needed when subcloning or modifying this vector if the number of transformants is low.
Proper citation: RRID:Addgene_22075 Copy
Species: Homo sapiens
Genetic Insert: partial sequence of human albumine
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22037 Copy
Species: Homo sapiens
Genetic Insert: hBEN
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11438732
Comments: GFP cloned in SpeI/ClaI
Proper citation: RRID:Addgene_22158 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10854432
Comments: Restriction sites may not be correct.
Proper citation: RRID:Addgene_22154 Copy
Species: Homo sapiens
Genetic Insert: mVenus-PA-Rac1-C450M
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBabe-TetCMV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19693014
Comments: Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.
Proper citation: RRID:Addgene_22034 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10854432
Comments: Restriction sites may not be correct.
Proper citation: RRID:Addgene_22153 Copy
Species: Homo sapiens
Genetic Insert: Epsin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10567358
Proper citation: RRID:Addgene_22242 Copy
Species: Homo sapiens
Genetic Insert: vexGFP_2A_OCT4
Vector Backbone Description: Backbone Size:6655; Vector Backbone:N/A; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19549847
Proper citation: RRID:Addgene_22240 Copy
Species: Homo sapiens
Genetic Insert: cyclin-dependent kinase inhibitor 2A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2284; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_22257 Copy
Species: Homo sapiens
Genetic Insert: HRAS G12V
Vector Backbone Description: Backbone Size:8378; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22259 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: No sequence information provided.
Proper citation: RRID:Addgene_22251 Copy
Species: Homo sapiens
Genetic Insert: HRAS G12V
Vector Backbone Description: Backbone Size:2466; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_22252 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9671454
Proper citation: RRID:Addgene_22188 Copy
Species: Homo sapiens
Genetic Insert: hBEN
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14645227
Comments: Restrictions sites may be incorrect.
Proper citation: RRID:Addgene_22183 Copy
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