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Genetic Insert: TP901-1 Integrase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179110 Copy
Species: Synthetic
Genetic Insert: GST-His8-GFP-nanobody
Vector Backbone Description: Backbone Marker:Cytiva; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178975 Copy
Species: Homo sapiens
Genetic Insert: FOXO3a
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pGex 4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10102273
Comments: See author's sequence and map.
Proper citation: RRID:Addgene_1790 Copy
Species: Synthetic
Genetic Insert: Sec61b-V5-TurboID
Vector Backbone Description: Backbone Marker:Tong-Chuan He laboratory; Backbone Size:35725; Vector Backbone:pAdTrack-CMV, pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34471136
Comments: Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971.
To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14.
Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.
Proper citation: RRID:Addgene_179143 Copy
Species: Danio rerio
Genetic Insert: Glial fibrillary acidic protein promoter
Vector Backbone Description: Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28882119
Proper citation: RRID:Addgene_178963 Copy
Species: Danio rerio
Genetic Insert: Glial fibrillary acidic protein promoter
Vector Backbone Description: Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28882119
Proper citation: RRID:Addgene_178964 Copy
Species: Synthetic
Genetic Insert: ABE8e
Vector Backbone Description: Vector Backbone:pRDA_256; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179097 Copy
Species: Synthetic
Genetic Insert: SpRY
Vector Backbone Description: Vector Backbone:pRDA_085; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179094 Copy
Species: Homo sapiens
Genetic Insert: shFEN
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18394896
Comments: Target sequence: TCACTAAGCAGCACAATGAT
Proper citation: RRID:Addgene_17921 Copy
Species: Synthetic
Genetic Insert: BE3.9 (Cas9-NG)
Vector Backbone Description: Vector Backbone:pRDA_256; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179095 Copy
Species: Synthetic
Genetic Insert: ABE8e (Cas9-NG)
Vector Backbone Description: Vector Backbone:pRDA_426; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179098 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446558
Proper citation: RRID:Addgene_179131 Copy
Species: Synthetic
Genetic Insert: ABE8e (SpG)
Vector Backbone Description: Vector Backbone:pRDA_426; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35288574
Proper citation: RRID:Addgene_179099 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446558
Proper citation: RRID:Addgene_179132 Copy
Species: Homo sapiens
Genetic Insert: WWP1 F792D
Vector Backbone Description: Backbone Marker:GEHealthcare; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16C
Proper citation: RRID:Addgene_179328 Copy
Species: Homo sapiens
Genetic Insert: H2B-mIFP-T2A-rTetR-HDAC4
Vector Backbone Description: Vector Backbone:pPB (PiggyBac); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179440 Copy
Species: Homo sapiens
Genetic Insert: H2B-mIFP-T2A-rTetR-KRAB
Vector Backbone Description: Vector Backbone:pPB (PiggyBac); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35678392
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.04.467237v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179439 Copy
Species: Homo sapiens
Genetic Insert: ELMSAN1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28C (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34912035
Comments: The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.
Proper citation: RRID:Addgene_179391 Copy
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:7304; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32984337
Proper citation: RRID:Addgene_179271 Copy
Species: Clostridium cellulosi
Genetic Insert: cellodextrin phosphorylase
Vector Backbone Description: Backbone Size:3464; Vector Backbone:self assembled; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35397553
Proper citation: RRID:Addgene_179276 Copy
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