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Genetic Insert: H2B
Vector Backbone Description: Backbone Marker:Michael Davidson Lab; Backbone Size:3900; Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879144
Comments: The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.
Proper citation: RRID:Addgene_74258 Copy
Species: Rattus norvegicus
Genetic Insert: Arrestin3
Vector Backbone Description: Backbone Marker:gift from M. Bouvier (University of Montreal); Vector Backbone:pcDNA3.1-RLucC2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27007854
Proper citation: RRID:Addgene_74129 Copy
Species: Homo sapiens
Genetic Insert: SMCR8
Vector Backbone Description: Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74158 Copy
Species: Homo sapiens
Genetic Insert: WDR41
Vector Backbone Description: Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74159 Copy
Genetic Insert: Connexin 43
Vector Backbone Description: Backbone Marker:Michael Davidson Lab; Backbone Size:3900; Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26879144
Comments: The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.
Proper citation: RRID:Addgene_74260 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:clontech/invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74315 Copy
Species: Danio rerio
Genetic Insert: zebrafish PSD95
Vector Backbone Description: Backbone Marker:Clontech/Invitrogen; Backbone Size:5; Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14758365
Comments: The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74314 Copy
Species: Danio rerio
Genetic Insert: synaptophysin
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16571769
Proper citation: RRID:Addgene_74317 Copy
Species: Mus musculus
Genetic Insert: treacle ribosome biogenesis factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4728; Vector Backbone:pS65T-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9811939
Comments: Please note that the amino acid sequence SGEFCSRRYRGPGIHRI is inserted after residue 1270 of treacle. The depositor noted that this sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_74297 Copy
Species: Mus musculus
Genetic Insert: treacle ribosome biogenesis factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4728; Vector Backbone:pS65T-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9811939
Proper citation: RRID:Addgene_74296 Copy
Species: Mus musculus
Genetic Insert: treacle ribosome biogenesis factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4728; Vector Backbone:pS65T-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9811939
Comments: Addgene Sanger sequencing determined that the treacle translation contains residue 1261.
Proper citation: RRID:Addgene_74298 Copy
Species: Mus musculus
Genetic Insert: mouse IKKε
Vector Backbone Description: Vector Backbone:pRFP.C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74414 Copy
Species: Mus musculus
Genetic Insert: Dedd
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pUC57-sgRNA expression vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74435 Copy
Species: Homo sapiens
Genetic Insert: human Hook3 amino acids 1-239, Q147A, M151A, I154A
Vector Backbone Description: Backbone Size:6198; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27482052
Proper citation: RRID:Addgene_74616 Copy
Species: Homo sapiens
Genetic Insert: human Hook3 amino acids 1-552
Vector Backbone Description: Backbone Size:6198; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27482052
Proper citation: RRID:Addgene_74614 Copy
Species: Lactobacillus brevis
Genetic Insert: mitoLbNOX
Vector Backbone Description: Backbone Size:2710; Vector Backbone:PUC57; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27124460
Comments: In our original publication we express mitoLbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this mitoLbNOX sequence into their preferred expression system.
Proper citation: RRID:Addgene_74448 Copy
Species: Homo sapiens
Genetic Insert: human Hook3 amino acids 1-239
Vector Backbone Description: Backbone Size:6198; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27482052
Proper citation: RRID:Addgene_74609 Copy
Species: Homo sapiens
Genetic Insert: human Hook3 amino acids 1-160
Vector Backbone Description: Backbone Size:6198; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27482052
Proper citation: RRID:Addgene_74607 Copy
Species: Homo sapiens
Genetic Insert: Neuropeptide Y
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:GFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74629 Copy
Species: Homo sapiens
Genetic Insert: BRD1A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|11321642
Proper citation: RRID:Addgene_74655 Copy
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