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Genetic Insert: RNAP alpha-Gal4
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACLU-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17406455
Comments: Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.
This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.
Proper citation: RRID:Addgene_13421 Copy
Species: Synthetic
Genetic Insert: kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31672972
Comments: pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134310 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression.
Proper citation: RRID:Addgene_13428 Copy
Species: Homo sapiens
Genetic Insert: mCherry-HJURP-LacI 1-304
Vector Backbone Description: Vector Backbone:pkg196; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134227 Copy
Species: Homo sapiens
Genetic Insert: DK360 tdTomato (long SKAP)
Vector Backbone Description: Backbone Marker:Erik Dent (Addgene plasmid # 50708); Vector Backbone:EB3-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134229 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134420 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV2; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134424 Copy
Genetic Insert: W4-loxP-TT-loxP-mRFP1
Vector Backbone Description: Backbone Size:2432; Vector Backbone:pBbA-k; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961670
Comments: Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint.
Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Proper citation: RRID:Addgene_134405 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134406 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134410 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134411 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134415 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134416 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134417 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Proper citation: RRID:Addgene_134418 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31433622
Comments: The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF.
Proper citation: RRID:Addgene_134419 Copy
Species: Homo sapiens
Genetic Insert: Rpp20
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26842893
Proper citation: RRID:Addgene_134541 Copy
Species: Homo sapiens
Genetic Insert: Rpp21
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26842893
Proper citation: RRID:Addgene_134542 Copy
Species: Homo sapiens
Genetic Insert: POP5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26842893
Proper citation: RRID:Addgene_134543 Copy
Species: Homo sapiens
Genetic Insert: Rpp25
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26842893
Proper citation: RRID:Addgene_134544 Copy
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