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Plasmids are provided by Addgene and DGRC.

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  • Bacterial Resistance:kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAC-Kan-alphaGal4
 
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RRID:Addgene_13421 RNAP alpha-Gal4 Kanamycin PMID:17406455 Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97. This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter. Backbone Size:3600; Vector Backbone:pACLU-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:36 0
pSiMPlk_N + pSiMPlk_C
 
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RRID:Addgene_134310 kanR_gp41-1 N-intein + kanR_gp41-1 C-intein Synthetic Kanamycin PMID:31672972 pSiMPlk_N should be cotransformed with pSiMPlk_C (or vice versa) in E. coli to get a reconstituted functional APT thereby conferring resistance against kanamycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlk_N + pSiMPlk_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:38 0
U6 pShuttle
 
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1+ mentions
RRID:Addgene_13428 Kanamycin pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression. Backbone Size:0; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin 2026-08-29 12:55:37 2
pKM510
 
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RRID:Addgene_134227 mCherry-HJURP-LacI 1-304 Homo sapiens Kanamycin Vector Backbone:pkg196; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin 2026-08-29 12:55:36 0
pDK713
 
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RRID:Addgene_134229 DK360 tdTomato (long SKAP) Homo sapiens Kanamycin Backbone Marker:Erik Dent (Addgene plasmid # 50708); Vector Backbone:EB3-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:37 0
S3_008_026_MV1
 
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RRID:Addgene_134420 mCherry Kanamycin PMID:31433622 The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF. Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:40 0
S3_007_026_MV2
 
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RRID:Addgene_134424 mCherry Kanamycin PMID:31433622 The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF. Vector Backbone:pYTKMV2; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:38 0
pBbAW4k-loxP-TT-loxP-mRFP1
 
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1+ mentions
RRID:Addgene_134405 W4-loxP-TT-loxP-mRFP1 Kanamycin PMID:31961670 Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint. Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers Backbone Size:2432; Vector Backbone:pBbA-k; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 12:55:38 1
S3_003_026_096
 
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RRID:Addgene_134406 mCherry Kanamycin PMID:31433622 Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:39 0
S3_007_026_096
 
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RRID:Addgene_134410 mCherry Kanamycin PMID:31433622 The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF. Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:38 0
S3_008_026_096
 
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RRID:Addgene_134411 mCherry Kanamycin PMID:31433622 The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF. Vector Backbone:pYTK096; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:39 0
S3_003_026_MV1
 
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RRID:Addgene_134415 mCherry Kanamycin PMID:31433622 Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:40 0
S3_004_026_MV1
 
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RRID:Addgene_134416 mCherry Kanamycin PMID:31433622 Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:38 0
S3_005_026_MV1
 
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RRID:Addgene_134417 mCherry Kanamycin PMID:31433622 Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:39 0
S3_006_026_096
 
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RRID:Addgene_134418 mCherry Kanamycin PMID:31433622 Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:40 0
S3_007_026_MV1
 
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RRID:Addgene_134419 mCherry Kanamycin PMID:31433622 The plasmid contains a small insertion 3' of the insert that results in the C-terminal NLS being unfused to the ORF. Vector Backbone:pYTKMV1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin none 2026-08-29 12:55:38 0
YFP-Rpp20-C1
 
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RRID:Addgene_134541 Rpp20 Homo sapiens Kanamycin PMID:26842893 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:39 0
YFP-Rpp21-C1
 
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RRID:Addgene_134542 Rpp21 Homo sapiens Kanamycin PMID:26842893 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:40 0
YFP-POP5-C1
 
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RRID:Addgene_134543 POP5 Homo sapiens Kanamycin PMID:26842893 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:41 0
YFP-Rpp25-C1
 
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RRID:Addgene_134544 Rpp25 Homo sapiens Kanamycin PMID:26842893 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-29 12:55:39 0

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