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Species: Homo sapiens
Genetic Insert: NFATc1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCR4; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16740479
Comments: The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007).
Proper citation: RRID:Addgene_23988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25
Vector Backbone Description: Backbone Size:5000; Vector Backbone:yEGFP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Entire RPL25 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3
Proper citation: RRID:Addgene_24037 Copy
Vector Backbone Description: Backbone Size:5099; Vector Backbone:pMOTag; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M.
Based on pMOTag series made in pBluescript II KS+
Proper citation: RRID:Addgene_24032 Copy
Genetic Insert: T7 RNA Polymerase
Vector Backbone Description: Backbone Size:6000; Vector Backbone:N/A; Vector Types:Trypanosome Expression; Bacterial Resistance:Ampicillin
Comments: T7 RNA Polymerase and Tet Repressor present (see map).
Target locus: Tubulin
Regulation: None
Proper citation: RRID:Addgene_24007 Copy
Species: Rattus norvegicus
Genetic Insert: ARMS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11150334
Comments: pFLAG-ARMS was generated by ligating two fragments of ARMS- HindIII/KpnI and KpnI/SpeI-- into the HindIII and XbaI sites of pFLAG. The HindIII site was generated by PCR;note that the SpeI and Xba I sites no longer exist after the ligation.
Proper citation: RRID:Addgene_24086 Copy
Species: Rattus norvegicus
Genetic Insert: TrkB
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11157096
Proper citation: RRID:Addgene_24088 Copy
Species: Rattus norvegicus
Genetic Insert: TrkC
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11157096
Proper citation: RRID:Addgene_24089 Copy
Vector Backbone Description: Backbone Marker:George Cross; Backbone Size:6145; Vector Backbone:pHD309-HYG; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Targeting: B-Tubulin
Regulation: None
For more information see George Cross's website: http://tryps.rockefeller.edu/trypsru2_plasmids.html
Proper citation: RRID:Addgene_24014 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pLEW100; Vector Types:Trypanosome Expression; Bacterial Resistance:Ampicillin
Comments: T7 terminators added: regulated rRNAp drives luciferase, giving about 10-fold higher te-induced expression in BF than pLEW100 with no increase in background.
Target locus: rRNA spacer
Regulation: 2 tet Op 2 T7 terminators
Proper citation: RRID:Addgene_24011 Copy
Species: Rattus norvegicus
Genetic Insert: TrkA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pDSRed2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_24093 Copy
Species: Mus musculus
Genetic Insert: Rorgt
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24069 Copy
Species: Mus musculus
Genetic Insert: IL23R
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: IL23R cDNA amplified through IL6 treated cDNA as 2 parts (5' part ATG-134-BamHI; 3' part 134-STOP).
5' part cloned into pcDNA3 XhoI/BamHI
3' part cloned into MSCV hCD2
Part linked together in pcDNA3 (XhoI-MluI)
Then cut with XhoI/NruI cloned into pMIGR (XhoI/HpaI)
Proper citation: RRID:Addgene_24066 Copy
Species: Mus musculus
Genetic Insert: Foxp3
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:7894; Vector Backbone:MSCV-LTRmiR30-PIG (LMP); Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18368049
Comments: The target sequence of Foxp3: 5′-GGCAGAGGACACTCAATGAAAT-3′
Proper citation: RRID:Addgene_24071 Copy
Species: Homo sapiens
Genetic Insert: HP1 alpha
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11959914
Proper citation: RRID:Addgene_24074 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16481433
Comments: The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.
Proper citation: RRID:Addgene_23999 Copy
Species: Rattus norvegicus
Genetic Insert: NR2A
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16481433
Comments: See attached map for additional plasmid features.
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.
Proper citation: RRID:Addgene_23997 Copy
Species: Rattus norvegicus
Genetic Insert: NR2B
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16481433
Comments: See attached map for additional plasmid features.
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.
Proper citation: RRID:Addgene_23998 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pRSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3471759
Comments: RSV - LacZ - STOP - SV40 PolyA
Proper citation: RRID:Addgene_24058 Copy
Species: Homo sapiens
Genetic Insert: Bmf
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pGSTag; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12591950
Proper citation: RRID:Addgene_24264 Copy
Species: Homo sapiens
Genetic Insert: RelA
Vector Backbone Description: Backbone Marker:Matthias et al, Nucleic Acid Research (1989).; Backbone Size:5120; Vector Backbone:modified pEV3S (pLG-033); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16135789
Comments: FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected])
Proper citation: RRID:Addgene_24156 Copy
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