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Genetic Insert: VP16AD-ZIP+
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pActPL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17088209
Comments: The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
Proper citation: RRID:Addgene_15305 Copy
Genetic Insert: Gal4AD-ZIP+
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pActPL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17088209
Comments: The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
Proper citation: RRID:Addgene_15303 Copy
Genetic Insert: sTurboID (C) (G74)-ERM (Cb5)
Vector Backbone Description: Backbone Size:8233; Vector Backbone:pLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32424107
Proper citation: RRID:Addgene_153007 Copy
Genetic Insert: sTurboID (N) (L73)
Vector Backbone Description: Backbone Size:7668; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32424107
Proper citation: RRID:Addgene_153002 Copy
Species: Synthetic
Genetic Insert: ABE7.10-nSpCas9
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152989 Copy
Species: Synthetic
Genetic Insert: eA3A-nSpCas9 NG-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152999 Copy
Species: Synthetic
Genetic Insert: eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152998 Copy
Species: Synthetic
Genetic Insert: eA3A-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152997 Copy
Species: Synthetic
Genetic Insert: BE4-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152991 Copy
Species: Synthetic
Genetic Insert: ABE7.10-nSpCas9 CP1041
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152990 Copy
Species: Synthetic
Genetic Insert: AID-nSpCas9-UGI-UGI
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32533916
Proper citation: RRID:Addgene_152994 Copy
Species: Homo sapiens
Genetic Insert: MKK4(ala)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7618106
Proper citation: RRID:Addgene_15314 Copy
Species: Drosophila melanogaster
Genetic Insert: Actin5c
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3500; Vector Backbone:pMT-V5/His'B'; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12975351
Proper citation: RRID:Addgene_15312 Copy
Species: Mus musculus
Genetic Insert: PRKACA
Vector Backbone Description: Backbone Marker:Dr. Richard Palmiter; Backbone Size:4100; Vector Backbone:Zem3 (pUC13 + MT prom + hGH polyA); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3667630
Comments: Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCalpha EV was constructed by isolating the 1318 base pair NaeI/PvuII fragment of pMCalpha and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA insert.
The coding sequence can be cut out with NcoI and ApaI, which should release a 1152bp fragment.
Proper citation: RRID:Addgene_15310 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: S-frag3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_153175 Copy
Species: Homo sapiens
Genetic Insert: human FOXA1
Vector Backbone Description: Backbone Size:4131; Vector Backbone:pCS2+ N-flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32858261
Proper citation: RRID:Addgene_153109 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153106 Copy
Species: Beggiatoa sp.
Genetic Insert: bPAC(WT)
Vector Backbone Description: Backbone Size:4136; Vector Backbone:pAAV CW3SL-eGFP (Addgene 61463); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037623
Proper citation: RRID:Addgene_153100 Copy
Species: Synthetic
Genetic Insert: NovArch
Vector Backbone Description: Backbone Size:9239; Vector Backbone:FCK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33952514
Proper citation: RRID:Addgene_153193 Copy
Genetic Insert: VP16AD-ZIP+
Vector Backbone Description: Backbone Size:13475; Vector Backbone:pCaST-elav; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17088209
Comments: The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.
Proper citation: RRID:Addgene_15308 Copy
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