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Species: E. coli
Genetic Insert: PTac-RBSQ1-QacR-PQacR-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74689 Copy
Species: E. coli
Genetic Insert: PTac-RBSP3-PhlF-PPhlF-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3930; Vector Backbone:pAN801; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74687 Copy
Species: Virus
Genetic Insert: Influenza A virus NS1 protein, partial
Vector Backbone Description: Backbone Marker:The Plant Journal (2006) 45, 616–629; Vector Backbone:pEarley-202; Vector Types:Plant Expression, double-stranded RNA labeling; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26351203
Proper citation: RRID:Addgene_74724 Copy
Species: Synthetic
Genetic Insert: RFP (BioBrick part number BBa_E1010)
Vector Backbone Description: Backbone Size:5316; Vector Backbone:pCM66T; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: One of a series of plasmids with promoters of differing strengths in Methylovorous glucosetrophus SIP3-4, a RuMP cycle methylotroph:
Addgene # 74738: empty vector, made from pCM66.
Addgene # 74739: pJM100: no-promoter RFP control. Made from pCM66D (AddGene #74738)
Addgene # 74740: pJM101: pHps1 RFP on pCM66T
Addgene # 74741: pJM102: pHps2 RFP on pCM66T
Addgene # 74744: pJM103: 150bp Hps2 RFP on pCM66T
Addgene # 74745: pJM104: pTrc RFP on pCM66T
Addgene # 74746: pJM105: pTac RFP on pCM66T
Backbone is similar backbone to pAWP78 (AddGene plasmid 61263)
Proper citation: RRID:Addgene_74741 Copy
Species: Synthetic
Genetic Insert: RFP (BioBrick part number BBa_E1010)
Vector Backbone Description: Vector Backbone:pCM66T (Addgene #74738); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: One of a series of plasmids with promoters of differing strengths in Methylovorous glucosetrophus SIP3-4, a RuMP cycle methylotroph:
Addgene # 74738: empty vector, made from pCM66.
Addgene # 74739: pJM100: no-promoter RFP control. Made from pCM66D (AddGene #74738)
Addgene # 74740: pJM101: pHps1 RFP on pCM66T
Addgene # 74741: pJM102: pHps2 RFP on pCM66T
Addgene # 74744: pJM103: 150bp Hps2 RFP on pCM66T
Addgene # 74745: pJM104: pTrc RFP on pCM66T
Addgene # 74746: pJM105: pTac RFP on pCM66T
Backbone is similar backbone to pAWP78 (AddGene plasmid 61263)
Proper citation: RRID:Addgene_74744 Copy
Species: Mus musculus
Genetic Insert: Eps8
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20532239
Proper citation: RRID:Addgene_74891 Copy
Species: Homo sapiens
Genetic Insert: Abi1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15048123
Proper citation: RRID:Addgene_74905 Copy
Species: Mus musculus
Genetic Insert: Eps8
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20532239
Proper citation: RRID:Addgene_74950 Copy
Species: Synthetic
Genetic Insert: ZIKV_sensor_32B_LacZ
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pCOLA Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27160350
Proper citation: RRID:Addgene_75007 Copy
Species: Synthetic
Genetic Insert: ZIKV_sensor_27B_LacZ
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pCOLA Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27160350
Proper citation: RRID:Addgene_75006 Copy
Species: Escherichia coli
Genetic Insert: phosphoribosylglycinamide synthetase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Constructed by Danny Crawford and T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).
In JM109 strain.
Proper citation: RRID:Addgene_75077 Copy
Species: Danio rerio
Genetic Insert: macrophage expressed 1, tandem duplicate 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75023 Copy
Species: Danio rerio
Genetic Insert: ELAV like neuron-specific RNA binding protein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75025 Copy
Species: E. coli
Genetic Insert: AcpP
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pet29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17184132
Proper citation: RRID:Addgene_75016 Copy
Species: Synthetic
Genetic Insert: EGFP-NS-MCS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2635; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75180 Copy
Species: Synthetic
Genetic Insert: H2B-mCerulean3-P2A-mKOFP2-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2647; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75177 Copy
Species: Synthetic
Genetic Insert: mTagBFP-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2656; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75176 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2666; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75175 Copy
Genetic Insert: Multiple Cloning Site
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2646; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75174 Copy
Species: Staphylococcus aureus
Genetic Insert: sortase A pentamutant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21697512
Comments: This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_75144 Copy
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